大豆灰斑病菌菌丝块离体叶接种快速鉴定方法的建立 |
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引用本文: | 张彪,黄军艳,刘胜毅,赵美霞,汪承刚,董彩华. 大豆灰斑病菌菌丝块离体叶接种快速鉴定方法的建立[J]. 安徽农业科学, 2011, 39(21): 12714-12718,12723 |
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作者姓名: | 张彪 黄军艳 刘胜毅 赵美霞 汪承刚 董彩华 |
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作者单位: | 中国农业科学院油料作物研究所,农业部油料作物生物学重点开放实验室,湖北武汉430062 |
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基金项目: | 大豆转基因专项,中国农业科学院油料研究所所长基金项目 |
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摘 要: | [目的]建立一种室内可重复、快速准确鉴定大豆对灰斑病抗性的方法。[方法]利用大豆灰斑病1号和7号生理小种及6种不同抗性的大豆品种为材料,研究并建立了大豆灰斑病抗性的菌丝块接种快速鉴定方法。[结果]在28℃、12h光照与12h黑暗交替的条件下,Minimal培养基生长的菌丝块接种抗感大豆叶片,菌斑面积明显不同,且鉴定结果与已知抗感品种报道的灰斑病抗性一致。[结论]菌丝块离体叶接种鉴定具有不受生育期和环境条件限制、可重复性强的特点,是大豆灰斑病抗性室内鉴定的一种有效方法。
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关 键 词: | 大豆灰斑病 菌丝块 离体叶 接种 抗性 |
Establishment of a Rapid Identification Method to Determine Differences in Susceptibility of Cercospora Sojina Hara by Hyphae Inoculated on the Excised Leaf Surface |
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Affiliation: | ZHANG Biao et al(Oil Crops Research Institute,Chinese Academy of Agricultural Sciences,Key Laboratory of Oil Crops Biology,Ministry of Agriculture,Wuhan,Hubei 430062) |
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Abstract: | [Objective]The aim was to establish a rapid identification method to determine differences in susceptibility of Cercospora Sojina Hara by hyphae inoculated on the excised leaf surface.[Method]6 varieties and 2 races(race 1and race 2) were used to study the establishment of a rapid identification method to determine differences in susceptibility of Cercospora Sojina Hara by hyphae inoculated on the excised leaf.[Result]The difference of lesion areas caused by the hyphae on Minimal medium was obvious under circumstances of 28 ℃ and 12 hlight per day,and the results were in sympathy with reports.[Conclusion]The hyphae inoculation method owns the features of non-growingseason restriction,non-circumstance restriction,simplicity and repeatability,so it could be an effective method in identifying the resistance to Cercospora Sojina Hara. |
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Keywords: | Cercospora Sojina Hara Hyphae Excised leaf surface Inoculation Susceptibility |
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