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(ACP5) in the Ovine Uterus: I. Regulation by Pregnancy and Progesterone
作者姓名:Haijun Gao  T.  E.  Spencer Guoyao  Wu  F.  W.  Bazer
作者单位:Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A&M University, College Station,TX,77843 ,USA
基金项目:The authors thank members of their laboratories for assistance with this research.
摘    要:Uteroferrin, also known as type 5 tartrate resistant acid phosphatase ( ACP5 ) or TRAP, is an iron-containing glycoprotein secreted by uterine gland epithelium (GE) in response to progesterone and transported across the placental areoalae into the fetal circulation and aUantoic fluid to deliver iron and to stimulate hematopoeisis in pigs. This study deter- mined if ACP5 was expressed in the ovine uterus in response to pregnancy, progesterone, interferon tau, placental lactogen, and placental growth hormone. ACP5 protein was present in uterine GE of cyclic and early pregnant ewes,particularly between days 18 and 120 of pregnancy. ACP5 mRNA was expressed in uterine GE of cyclic and pregnant ewes in the same temporal and cell-specific manner. ACP5 was present in secretions from uterine glands, i. e. , uterine milk,and allantoic fluid from days 40 to 80 of pregnancy, and in uterine flushings from cyclic and early pregnant ewes. Progesterone induced expression of ACP5 mR- NA and intrauterine infusion of recombinant ovine interferon tau further stimulated ACP5 expression in uterine GE of ewes,but intrauterine injections of ovine placental lactogen and ovine growth hormone had no effect on ACP5 expression in uterine GE. These re- sults indicate that ACP5 is:1 ) expressed only in GE in response to progesterone ;2) secreted into the uterine lumen and transported into the conceptus via placental areolae during pregnancy;and 3) present in secretions from uterine GE and in allantoic fluid. The roles of ACP5 in the ovine uterus may include transport of iron across the placenta and stimulation of hematopoiesis.

关 键 词:子宫冲洗  怀孕母羊  孕激素  绵羊  转铁蛋白  规例  蛋白分泌  生长激素
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