籼稻诱变糯质基因的回复突变

以辐射诱变籼型杂交稻亲本育成的糯质基因突变系南恢175wxR、湘恢288wxR、明恢86wxR、蜀恢527wxR、明恢63wxRI、I-32wxB、龙特浦wxB和D62wxB为材料,借助肉眼观察和1%KI-I2溶液鉴定各自交后代糙米粒胚乳性质,首先发现诱变糯质基因wx发生回复突变(即胚乳性质由糯质回复突变为非糯质),回复突变频率为0~6.34×10-5,平均为3.74×10-5。遗传分析表明,回复突变是显性突变。糯质基因突变系与糯质基因回复突变系和野生型亲本间除千粒重差异显著外,其他性状差异均不显著。提出杂交糯稻亲本原种生产及种子保纯技术。

THE MUTATION REVERSION OF INDUCED WAXY GENE OF RICE IN INDICA

Some induced waxy gene lines such as Nanhui 175wxR,Xianghui 288wxR,Minghui 86wxR,Shuhui 527wxR,Minghui 63wxR,II-32wxB,Longtepu wxB and D62wxB,which developed through irradiation induction of the parents of hybrid rice in indica,were selected as the materials,and the properties of endosperm of their inbred descendant brown rice were identified by visual estimation and by the method of 1% KI-I_2 determination.It was the first time to discover the reversion of induced waxy mutants(waxy endosperm reversed to non-waxy ones) in rice,and the reversion frequency was between 0 to 6.34×10~(-5),with a average of 3.74×10~(-5). Genetic analysis indicated that induced waxy mutants reversion gene was dominant.Except for 1000-grain weight,no significant difference in other traits was found among the induced waxy mutants,the waxy mutants reversion and the wilds.A new core seed production and seed purity control techniques of the parents of waxy hybrid rice was also put forward in this paper.