| 春兰SRAP-PCR反应体系的建立和优化 |
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| 引用本文： | 牛田,张林,王厚新,李承秀.春兰SRAP-PCR反应体系的建立和优化[J].农学学报,2013,3(11):25-29. |
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| 作者姓名： | 牛田 张林 王厚新 李承秀 |
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| 作者单位： | [1]山东农业大学林学院,山东泰安271018 [2]泰山林业科学院,山东泰安271000 |
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| 基金项目： | 国家公益专项“国兰新品种培育与产业化生产关键技术研究”（201004080）. |
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| 摘 要： | 摘 要：【研究目的】为获得春兰的 SRAP 标记图谱，对春兰SRAP-PCR 反应体系进行了初步研究【方法】通过正交试验设计,从Mg2+、dNTPs、Taq酶、引物、模板5种因素4个水平对春兰SRAP-PCR反应体系进行优化。【结果】所建立的体系为：25μl反应体系中含有2.5mmol/L的Mg2+ ，2mmol/L的dNTP, 0.5U的Taq酶,1.2 μmol/L的引物,90 ng的模板。【结论】为春兰指纹图谱的构建奠定基础。
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| 关 键 词： | 余甘子 余甘子 氨基酸 成分分析 |
| 收稿时间： | 2013/5/14 0:00:00 |
| 修稿时间： | 2013/6/19 0:00:00 |
Establishment and Optimization of SRAP-PCR Reaction System of Cymbidium goeringii |
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| Niu Tian,Zhang Lin,Wang Houxin,Li Chengxiu,Sun Fang,Yu Yongchang,Yan Tingmei,Wang Changxian.Establishment and Optimization of SRAP-PCR Reaction System of Cymbidium goeringii[J].Journal of Agriculture,2013,3(11):25-29. |
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| Authors: | Niu Tian Zhang Lin Wang Houxin Li Chengxiu Sun Fang Yu Yongchang Yan Tingmei Wang Changxian |
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| Institution: | 2 (College of Forestry, Shandong Agriculture University, Tai＇ an 271018, Shandong, China; 2Taishan Forestry Academe, Tai an 271000, Shandong, China) |
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| Abstract: | To obtain Sequence-related amplified polymorphism （SRAP） fingerprints of Cymbidium goeringii, the factors influencing the SRAP reaction system were studied. The major components of SRAP, such as concentrations of Mg2＊, dNTP, Taq DNA polymerase, primers and template, were optimized in this study by orthogonal design in five factors four levels respectively. The results showed that the optimum SRAP reaction system included Mg2＋ 2.5 mmol/L, dNTP 2 mmol/L, Taq DNApolymerase 0.5 U, primer 1.2 mmol/L and DNA template 90 ng in the total 25 p,L reaction system. It laid the foundation for the construction of fingerprints |
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| Keywords: | Cymbidium goeringii SRAP Orthogonal Design Optimization |
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