4个加工番茄新品种SSR指纹图谱的构建与品种鉴定

目的]探明加工番茄主要品种的遗传变异,建立准确高效的种子纯度鉴定方法.方法]利用SSR引物对4个加工番茄杂交品种及其8个亲本进行PCR扩增,经4;的变性聚丙烯酰胺凝胶电泳,进行带型分析.结果]从34对SSR引物中筛选出10对扩增产物具有稳定多态性的引物,这些引物分布在番茄整个基因组,每对引物可以检测到2～4个数目不等的多态性片段,共28个多态性片段,平均2.8个,片段大小介于60～150 bp,成功构建出4个加工番茄杂交种及其8个亲本的指纹图谱,并鉴定出了这4个杂交种的共显性标记.结论]这些标记不仅可用于亲本材料和杂交品种的真伪鉴别,还可为杂交种纯度的鉴定提供可靠的方法,对亲本知识权的保护及杂交种推广具有重要意义.

Construction of Fingerprint and Identification for Foux Processing Tomato Hybrids by SSR Marker

Objective]In order to identify the genetic diversity of tomato hybrids and establish the identification method for seed purity.Method]Four processing tomato hybrids and eight parent lines were screened for simple sequence repeat(SSR) primers.Gel electrophoresis of the amplified product was carried out with 4% polyacrylamide and further study on their polymorphmism.Result]Among 34 pairs of primers,10 pairs of steady polymorphism were selected and distributed in tomato genome.2 to 4 alleles were detected by each pair of primers.There were 28 polymorphism fragments in total,whose average number was 2.8 per pair of primer.The fragment size ranged from 60 to 150bp.And DNA fingerprinting of successfully constructed.In addition,codominant markers of the four hybrids were identified.Conclusion]These markers not only can be used to distinguish four processing tomato hybrids and eight parent lines between real and false parent materials or hybrids,but also to supply reliable methods for hybrids seed purity inspection,which is of great importance for the property protection of the parent lines and safe popularization of hybrid seeds.