Kunitz-type serine protease inhibitor from potato (Solanum tuberosum L. cv. Jopung) |
| |
Authors: | Park Yoonkyung Choi Bo Hwa Kwak Ji-Sun Kang Chang-Won Lim Hak-Tae Cheong Hyeon-Sook Hahm Kyung-Soo |
| |
Institution: | Research Center for Proteineous Materials, Department of Genetic Engineering, Chosun University, 375 Seosuk-Dong, Korea. |
| |
Abstract: | An antifungal protein, AFP-J, was purified from tubers of the potato (Solanum tuberosum cv. L Jopung) by various chromatographic columns. AFP-J strongly inhibited yeast fungal strains, including Candida albicans, Trichosporon beigelii, and Saccharomyces cerevisiae, whereas it exhibited no activity against crop fungal pathogens. Automated Edman degradation determined the partial N-terminal sequence of AFP-J to be NH2-Leu-Pro-Ser-Asp-Ala-Thr-Leu-Val-Leu-Asp-Gln-Thr-Gly-Lys-G lu-Leu-Asp-Ala-Arg-Leu-. The partially sequence had 83% homology with a serine protease inhibitor belonging to the Kunitz family, and the protein inhibited chymotrypsin, pepsin, and trypsin. Mass spectrometry showed that its molecular mass was 13 500.5 Da. This protease inhibitor suppressed over 50% the proteolytic activity at 400 microg/mL. These results suggest that AFP-J is an excellent candidate as a lead compound for the development of novel antiinfective agents. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|