黄瓜花药和花粉高温伤害与多胺和脯氨酸含量的关系

 黄瓜花药在开花期比花粉母细胞形成期(阶段1) 和单核花粉期(阶段2) 腐胺、精胺、亚精胺含量较低, 游离脯氨酸(Pro) 含量较高。高温(35 ℃/ 25 ℃) 使阶段1、阶段2 花药中上述3 种内源多胺含量及开花期花药和花粉中Pro 含量下降。黄瓜花粉在萌发培养基上培养1 h 时3 种内源多胺含量达峰值,此后下降; Pro 含量随着花粉萌发逐步下降; 高温(35 ℃) 下黄瓜花粉萌发后3 种内源多胺含量的上升和Pro 水平的下降趋势被抑制, 但耐热品系XC21、NY21 受高温的影响小于热敏品系新泰密刺或不受影响, 表明多胺和Pro 代谢与耐热性有关。外源鸟氨酸转氨酶(OAT) 的抑制剂3 氨基- 2 , 3 - 二羟基安息香酸(Gabaculine) 能降低黄瓜花粉的萌发能力, 同时提高花粉中内源多胺的含量并降低Pro 的含量, 表明黄瓜花粉萌发过程中可能存在由Pro 合成多胺的途径。

The Relationship Between Heat Injury and Polyamines or Proline Contents During Anther Development and Pollen Germination in Cucumber

Cucumber (Cucumis sativus L. ) anthers had lower Put , Spm, Spd contents and higher free proline (Pro) contents at anthesis than those at pollen mother cell formation stage and monokaryotic pollen stage, high temperature (35 ℃/ 20 ℃) could reduce Put , Spm , Spd contents at stage 1 and stage 2, and reduce Pro contents in anthers and pollens at anthesis. During cucumber pollen culturing, Put, Spm, Spd contents reached max in 1 h then descended, Pro contents descended gradually, Put, Spm, Spd contents decreased and Pro contents increased when cucumber pollen germinated under high temperature ( 35 ℃) . In heat sensitive line Xintaimici , the effects of high temperature on physiological processes above were more serious than those in heat tolerant line XC-1 and NY-1 , which indicated polyamine and Pro might play a key role in cucumber anther development and pollen germination. Gabaculine, a inhibitor of ornithine aminotransferase, could inhibit cucumber pollen germination, improve polyamine contents and reduce Pro contents during pollen germination, which indicated there might be a Pro to polyamine biochemistry route during cucumber pollen germination.