| 高抑制金黄色葡萄球菌活性放线菌的分离及分子鉴定 |
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| 引用本文： | 窦会娟,李林珂,刘新胜,孙连海.高抑制金黄色葡萄球菌活性放线菌的分离及分子鉴定[J].安徽农业科学,2011(9):5076-5077. |
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| 作者姓名： | 窦会娟 李林珂 刘新胜 孙连海 |
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| 作者单位： | 漯河医学高等专科学校基础医学部; |
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| 基金项目： | 河南省教育厅自然科学基金项目(2010C180005) |
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| 摘 要： | 从医院土壤中筛选分离代谢物具有较高抑制金黄色葡萄球菌活性的放线菌,并对其进行分子鉴定。结果得出,采用影印平皿法,以金黄色葡萄球菌为指示菌进行放线菌的分离,通过16S rDNA序列分析进行鉴定。结果得出,A01菌株发酵液对金黄色葡萄球菌抑制效果较好,与Streptomyces sp.VTTE-042674亲缘关系最近,达到99.86%,应属于链霉菌属。该菌株的16S rDNA序列已被GenBank收录,序列登录号为HQ660584。
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| 关 键 词： | 分离 分子鉴定 16S rDNA 系统发育分析 |
Isolation and Molecular Identification of Actinomycetes That Highly Inhibited the Activity of S.aureus |
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| DOU Hui-juan et al.Isolation and Molecular Identification of Actinomycetes That Highly Inhibited the Activity of S.aureus[J].Journal of Anhui Agricultural Sciences,2011(9):5076-5077. |
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| Authors: | DOU Hui-juan |
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| Institution: | DOU Hui-juan et al(Department of Basic Medicine,Luohe Medical College,Luohe,Henan 462002) |
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| Abstract: | Metabolin was screened and isolated from hospital soil,which was the actinomycete with highly inhibition for S.aureus activity,and molecular identification was carried out for actinomycete.Photocopy dish was used,and Staphylococcus aureus was viewed as indicator bacteria for the isolation of actinomycetes,then carrying out identification based on 16S rDNA sequence analysis.The results indicate that A01 bacterial strain fermentation broth have better inhibition effect on S.aureus,and have good relationship w... |
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| Keywords: | Isolation Molecular identification 16S rDNA Phylogenetic analysis |
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