| 家蚕TPR基因的克隆 |
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| 引用本文： | 牛伟涛. 家蚕TPR基因的克隆[J]. 安徽农业科学, 2011, 39(9): 5114-5115 |
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| 作者姓名： | 牛伟涛 |
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| 作者单位： | 邢台学院生化系,河北邢台,054001 |
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| 摘 要： | [目的]克隆家蚕TPR基因,为研究该基因功能提供依据。[方法]利用Trizol法提取家蚕蛹期总RNA,再用RT-PCR方法对家蚕TPR基因进行克隆。[结果]成功得到家蚕TPR基因全cDNA序列,并用PCR扩增得到了TPR基因的ORF,全长858 bp。[结论]首次对家蚕TPR家族基因进行了克隆,为今后对该基因功能研究奠定了坚实的基础。
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| 关 键 词： | RNA的提取 PCR技术 基因克隆 |
Cloning of TRP Gene of Bombyx mori |
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| NIU Wei-tao. Cloning of TRP Gene of Bombyx mori[J]. Journal of Anhui Agricultural Sciences, 2011, 39(9): 5114-5115 |
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| Authors: | NIU Wei-tao |
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| Affiliation: | NIU Wei-tao(Department of Biochemistry,Xingtai University,Xingtai,Hebei 054001) |
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| Abstract: | [Objective] The aim was to clone Bombyx mori TRP gene.[Method] The total RNA of Bombyx mori was extracted during its pupal period with Trizol method,and then the TRP gene was cloned by RT-PCR.[Result] The full-length cDNA of TRP gene was successfully obtained,and the ORF fragment(858 bp) of TRP gene was cloned by PCR.[Conclusion] TRP gene of Bombyx mori was cloned for the first time,which could provide solid basis for the study on its function. |
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| Keywords: | RNA extraction PCR technology Gene cloning |
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