| 冬虫夏草菌种油混悬剂的研究 |
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| 引用本文： | 刘洪波,王贵学,朱华李,陈仕江,毛先兵. 冬虫夏草菌种油混悬剂的研究[J]. 中国生物防治, 2008, 0(Z1) |
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| 作者姓名： | 刘洪波 王贵学 朱华李 陈仕江 毛先兵 |
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| 作者单位： | 重庆大学生物工程学院;重庆市中药研究院; |
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| 基金项目： | 国家科技支撑计划项目(2006BAI06A13)的部分研究内容 |
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| 摘 要： | 冬虫夏草人工培育中,接种侵染成功率一直是制约其规模化生产的重要因素。本次试验采用正交试验设计,应用数学软件MATLAB极差分析法,根据综合平衡法原则,筛选出冬虫夏草菌种油混悬剂最优的组合方案,即菜籽油与邻苯二甲酸二丁酯(7:3)复合油10g,固液比1.0:10,十二烷基硫酸钠0.9g、脂肪醇聚氧乙烯醚0.5g、硬脂酸铝0.6g,其制剂的沉降体积比和再分散性分别高达97%和100%。高活力菌丝和孢子制成的油混悬剂提高了侵染成功率,适宜作为人工培育冬虫夏草的菌种,能满足大规模培育冬虫夏草的接种需求。
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| 关 键 词： | 冬虫夏草 中国被毛孢 油混悬剂 菌种 |
Oil Miscible Flowable Concentrate of Cordyceps sinensis Spawn |
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| LIU Hong-bo,WANG Gui-xue,ZHU Hua-li,CHEN Shi-jiang,MAO Xian-bing. Oil Miscible Flowable Concentrate of Cordyceps sinensis Spawn[J]. Chinese Journal of Biological Control, 2008, 0(Z1) |
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| Authors: | LIU Hong-bo WANG Gui-xue ZHU Hua-li CHEN Shi-jiang MAO Xian-bing |
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| Affiliation: | LIU Hong-bo~(1,2),WANG Gui-xue~1,ZHU Hua-li~2,CHEN Shi-jiang~2,MAO Xian-bing~(2*) (1.Bioengineering College of Chongqing University,Chongqing 400044,China,2.Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China) |
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| Abstract: | During the cultivation of Cordyceps sinensis,the successful invading hosts by Hirsutella sinensis is always one of the key factors of limiting its commercialization.In this experiment,we screened the optimal combination project of oil miscible flowable concentrate of H.sinensis by orthogonal design, range analysis of MATLAB software and the principle of comprehensive balance,namely,a mixture of rapeseed oil and dibutyl phthalate(7:3) 10g,K120.9g,fatty alcohol polyoxyethylene ether 0.5 g,aluminium stearate 0... |
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| Keywords: | Cordyceps sinensis Hirsutella sinensis oil miscible flowable concentrate spawn |
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