旋毛虫排泄-分泌抗原的制备及在免疫学诊断中的应用研究

目的:将排泄-分泌抗原用于Dot-ELISA免疫学诊断试验,检测其抗原特异性和敏感性。方法经培养24h、48h、72 h排泄-分泌抗原ES1、ES2、ES3,对38头份猪旋毛虫病阳性猪血清、98头猪旋毛虫阴性血清、26头猪囊虫阳性血清、35头猪蛔虫阳性血清和21头猪细颈囊尾蚴阳性血清进行了Dt-ELISA免疫学方法检测并于粗抗原进行比较。结果排泄-分泌抗原的特异性为ES1(99.44%)、ES2(96.11%)、ES3(92.77%),敏感性ES1(97.36%)、ES2(92.11%)、ES3(84.21%)。结论排泄-分泌抗原ES1(培养24小时)抗原特异性强,敏感性高,重复性好,用于旋毛虫病的免疫诊断有重要作用。

Study on Trichina Spiralis ES Antigens Preparation and Exploratory Development by Immunology Assay

Objective To detect Trichina spiralls ES antigenic specificity and sensitivity with Dot-ELISA..Methods Inoculate Es1,Es2 and Es3 on 24 hour,48 hour and 72 hour,detecting and comparing 38 masculine blood-serums from Trichina spiralls,98 negative blood-serums from Trichina spiralls,26 masculine blood-serums from bladder worm,35 masculine blood-serums from Ascaris suilla and 21 masculine blood-serums from cysticercus tenuicollis with Dot-ELISA.Results The result showed that specificity and sensitivity,Es1 was 99.44% and 97.36%,Es2 was 96.11% and 92.11%,Es3 was 92.77% and 84.21%.Conclusion Es1 from Trichina spiralls is high antigenic and sensitivity,better reproducibility,and which is important by immunology Assay.