| 不同提取条件对葡萄叶提取物抑制两种病原菌活性的影响 |
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| 引用本文： | 袁茜,周宝利,李之璞.不同提取条件对葡萄叶提取物抑制两种病原菌活性的影响[J].植物保护,2009,35(2):125-127. |
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| 作者姓名： | 袁茜 周宝利 李之璞 |
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| 作者单位： | 1. 沈阳农业大学园艺学院,沈阳,110161
2. 沈阳市农业科学院,沈阳,110034 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 以黄瓜枯萎病菌和辣椒枯萎病菌作为供试菌种，采用生长速率法对葡萄叶不同溶剂提取物和不同提取方法提取物的抑菌活性进行测定。结果表明，葡萄叶不同溶剂提取物对供试菌种均表现出抗菌效果，并筛选出最佳提取溶剂是95%的乙醇，而最佳提取条件是提取温度40 ℃、提取时间和方式是超声波提取30 min，提取料液比1∶8(g/mL)。
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| 关 键 词： | 葡萄叶 植物病原菌 抑菌活性 |
Effects of the extracts from Vitis amurensis leaves with different methods on Fusarium oxysporum f.sp.cucumerinum and F.oxysporum f.sp.vasinfectum |
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| Yuan Qian,Zhou Baoli,Li Zhipu.Effects of the extracts from Vitis amurensis leaves with different methods on Fusarium oxysporum f.sp.cucumerinum and F.oxysporum f.sp.vasinfectum[J].Plant Protection,2009,35(2):125-127. |
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| Authors: | Yuan Qian Zhou Baoli Li Zhipu |
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| Institution: | 1. College of Horticulture, Shenyang Agricultural University, Shenyang110161, China;2. Shenyang Agricultural Institute, Shenyang110034, China |
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| Abstract: | Fusarium oxysporum f.sp. cucumerinum and F. oxysporum f.sp. vasinfectum were selected for test. The antifungal activity of leaf extracts of Vitis amurensis in different solvents and different extraction methods were tested by using growth rate method. The results showed that different extracts from different solvents all had antifungal activity, and 95% ethanol were selected as the best solvent. The optimum conditions were determined to be 40 ℃, ultrasonic treatment for 30min, 1∶8 (g/mL) extraction ratio. |
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| Keywords: | Vitis amurensis leaves phytopathogens antifungal activity |
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