Characteristics of the whole cell fatty acid profiles ofPseudomonas corrugate |
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| Authors: | Felipe Siverio Emilio A. Carbonell Francesc García María M. López |
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| Affiliation: | (1) Instituto Canario de Investigaciones Agrarias (ICIA), Apartado de Correos 60, 38200 La Laguna, Tenerife, Spain;(2) Instituto Valenciano de Investigaciones Agrarias (IVIA), Apartado Oficial, 46113 Moncada, Valencia, Spain;(3) Servei de Protecció dels Vegetals, Ctra. de Vilasar de Mar s/n, 08348 Cabrils, Barcelona, Spain |
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| Abstract: | The fatty acid methyl ester (FAME) profiles of eighty strains ofPseudomonas corrugata from different geographic origins have been studied. Gas chromatographic profiles were obtained. The use of hexane/methyl-tert butyl ether (MTBE) (1 1) for the extractions improves the yield of hydroxy-FAMEs used to identifyP. corrugata as compared to hexane alone. The analysis of the extracts with hexane/MTBE showed that many strains do not have the characteristic FAMEs ofP. corrugata (3-hydroxydodecenoic acid, 3-hydroxytetradecanoic acid, 3-hydroxyhexadecanoic acid and two unknown additional fatty acids). These differences among strains seemed to be related to bacterial dissociation, associated with changes in morphological aspect of the colonies due to subculture. The comparison of profiles of wrinkled and smooth colonies isolated from ten strains confirmed the differences among those in specific FAMEs. Therefore, the FAME profiles are a useful tool for the identification ofP. corrugata when the bacteria have not been subculturedin vitro for a long time. Multivariate analyses of data showed that four clusters can be observed supporting the heterogeneity of the strains ofP. corrugata.Abbreviations FAME fatty acid methyl ester - MTBE methyl tert-butyl ether |
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| Keywords: | Pseudomonas corrugata fatty acids identification |
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