| 不同分子量大豆多糖的表征和抗氧化研究 |
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| 引用本文： | 何喜珍,牛延宁,金明飞,黄静,崔红亮,常忠义,高红亮. 不同分子量大豆多糖的表征和抗氧化研究[J]. 大豆科学, 2016, 0(5): 805-809. DOI: 10.11861/j.issn.1000-9841.2016.05.0805 |
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| 作者姓名： | 何喜珍 牛延宁 金明飞 黄静 崔红亮 常忠义 高红亮 |
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| 作者单位： | 1. 华东师范大学生命科学学院,上海,200241;2. 平顶山金晶生物科技有限公司,河南平顶山,467215 |
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| 基金项目： | 国家自然科学基金(31170920). |
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| 摘 要： | 以酸提大豆多糖为原料,通过控制降解条件得到4种不同分子量的大豆多糖样品,分子量分别为550,347,285和21 k Da。对4种多糖进行了傅里叶红外(FT-IR)分析和X射线衍射(XRD)分析,并分别测定了4种多糖对DPPH自由基、羟基自由基的清除能力以及还原能力的大小,结果表明:4种大豆多糖都有一定的抗氧化活性,但抗氧化活性高低有所不同,分子量为285 k Da的大豆多糖对自由基的清除能力最强,还原能力最强,分子量为21 k Da的大豆多糖对自由基的清除能力最弱,还原能力最弱。
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| 关 键 词： | 大豆多糖 降解 抗氧化 表征 |
Characterization and Antioxidation Study of Different Molecular Weight of Soluble Soybean Polysaccharides |
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| Abstract: | Four different molecular-weight soluble soybean polysaccharides were prepared by controlling the degration condition,with the molecular weights 550,347,285 and 21 kDa,respectively.The structures of them were identified by Fourier transform infrared spectrometer (FTIR) and X-Ray Diffraction (XRD).Antioxidant activities of four SSPS were evaluated in vitro by DPPH free radical scavenging capacity,hydroxyl free radical scavenging and total reducing power.The results showed that all of them had antioxidant activities.The SSPS-H with molecular weight of 285 kDa had the strongest antioxidant activities in all antioxidant systems,while the SSPS-CH with molecular weight of 21 kDa performed the worst. |
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| Keywords: | Soluble soybean polysaccharides Degration Antioxidant Characterization |
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