石鲽脑垂体组织生理学研究

石鲽(Kareius bicoloratus)脑垂体呈鸡心形,由神经垂体和腺垂体两部分组成。腺垂体又包括前外侧部(RPD)、中外侧部(PPD)和垂体中间部(PI)。神经纤维束延伸至腺垂体的各个部分。采用HE、Jafri、HA、Mallory和PAS染色法,结合免疫组织化学方法,对石鲽脑垂体的6种内分泌细胞进行鉴别和定位。RPD部位主要含有PRL和ACTH细胞,PI部位主要含有MSH细胞。PPD部位有4类细胞,分别为GH、Gt H、TSH和嫌色细胞。GH、Gt H和TSH细胞数量随鱼体生长发育而变化,Gt H在性腺成熟期达到高峰,GH细胞和TSH细胞在生长早期数量多。

Studies on histological physiology of the pituitary in stone flounder (Kareius bicoloratus)

Composed of neurohypophysis and adnohypophysis, the pituitary in stone flounder (Kareius bicoloratus) assumes the shape similar to the heart of chicken. Adnohypophysis could be divided into rostral pars distails (RPD), proximal pars distails (PPD) and pars intermedia (PI). Nerve fibre extends to every part of adnohypophysis. By using H.E, Jafri, HA, PAS, and Mallory staining techniques and immunohistochemical methods, the six kinds of endocrine cells in adnohypophysis are identified and located. RPD mainly contains a mass of PRL and ACTH cells and a few TSH cells, while PI contains mostly MSH cells. In PPD, there are four kinds of endocrine cells, including GH, GtH, TSH and Chromophpbe cells. Most endocrine cells keep changing with the development of the gonad in quantity, especially GH, GtH and TSH cells. GtH cells reach the highest value in gonad maturation, whereas GH cells and TSH cells achieve the amount peak in pre-reproductive season.