| 猪繁殖与呼吸道综合征病毒(PRRSV)单克隆抗体的研制 |
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| 引用本文： | 韩庆安,姜平,董永毅. 猪繁殖与呼吸道综合征病毒(PRRSV)单克隆抗体的研制[J]. 中国兽医学报, 2000, 20(2): 145-147 |
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| 作者姓名： | 韩庆安 姜平 董永毅 |
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| 作者单位： | [1]南京农业大学农业部动物疫病诊断与免疫重点实验室 [2]河北农业大学动物科技学院 |
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| 基金项目： | 中华农业科教基金，江苏省“九五”重点攻关项目!( BE964 0 4) |
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| 摘 要： | 利用猪繁殖与呼吸道综合征病毒（ＰＲＲＳＶ）国内分离株Ｊ１，采用反复差速离心法制备免疫抗原，长程免疫法免疫ＢＡＬＢ／ｃ小鼠，用间接ＥＬＩＳＡ方法检测抗体，通过细胞融合技术，并经３次亚克隆获得了１０株能稳定分泌抗ＰＲＲＳＶ单抗的杂交瘤细胞单克隆株（Ａ１Ｄ７Ｈ１０，Ａ１Ｄ７Ｈ１１，Ａ１Ｅ７Ｈ９，Ａ１Ｅ７Ｄ９，Ａ２Ｄ８Ｅ７，Ａ２Ｄ８Ｂ１１，Ｂ３Ｄ１１Ｄ６，Ｂ２Ｇ９Ａ９，Ｂ２Ｇ９Ｆ２）。这些细胞经体外连续传
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| 关 键 词： | PRRSV 单克隆抗体 杂交瘤细胞株 腹水效价 |
Development of the monoclonal antibodies against porcine reproductive and respiratory syndrome virus(PRRSV) |
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| HAN Qing-an,JIANG Ping,DONG Yong-yi. Development of the monoclonal antibodies against porcine reproductive and respiratory syndrome virus(PRRSV)[J]. Chinese Journal of Veterinary Science, 2000, 20(2): 145-147 |
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| Authors: | HAN Qing-an JIANG Ping DONG Yong-yi |
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| Abstract: | Indirect enzyme linked immunosorbent assay(ELISA) method was developed to detect antibodies against PRRSV with J 1 isolated from the Huabei region of China. The viral antigens were purified by a number of centrifugations at low speed and ultraspeed. After the BALB/c mice to be immunized five times,the secreting antibody hybridomas were obtained.By screening with ELISA and cloning three times using the method of limiting dilution,ten hybridoma cell lines with a high and stable level of antibodies secreted were developed. The monoclonal antibodies were produced in cell culture supernatants or in ascites. The ELISA titer of the ascites was 10 -3 10 -4 .Among them,seven hybridoma cell lines of monoclonal antibodies of PRRSV J 1 strain could be bound to Rerp PRRSV strain. |
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| Keywords: | PRRSV monoclonal antibody hybridoma cell line titer of ascites |
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