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| 花生子叶遗传转化再生体系影响因素的研究 | |
| 引用本文： | 刘峰,单世华,闫彩霞,毕玉平,李光敏,伦伟志.花生子叶遗传转化再生体系影响因素的研究[J].花生学报,2007,36(1):13-19. |
| 作者姓名： | 刘峰 单世华 闫彩霞 毕玉平 李光敏 伦伟志 |
| 作者单位： | 1. 山东师范大学生命科学学院,山东,济南,250014;山东省农科院高新技术研究中心,山东,济南,250100 2. 山东省花生研究所,山东,青岛,266100 3. 山东省农科院高新技术研究中心,山东,济南,250100 4. 山东省莒县陵阳镇农技站,山东,莒县,276500 5. 山东省莒县城阳镇农技站,山东,莒县,276500 |
| 基金项目： | 山东省青岛市科技局资助项目 , 山东省农科院创新基金 |
| 摘 要： | 本试验选用子叶为外植体研究了影响植株再生和遗传转化的因素。结果表明,细胞分裂素(6-BA)5.00 mg/L (2,4-D)1.50 mg/L的诱导培养基子叶外植体丛生芽诱导率最高,分别达89.67%和83.33%。遗传转化影响因素结果表明,菌液OD600为0.5-0.7、侵染时间10min、共培养时间3d、每50mL菌液添加烟草提取物1.0mL可获得较好的转化效果。抗生素对芽分化率影响的研究结果表明,当头孢唑林钠(Cef)250 mg/L 羧苄青霉素(Carb)250-500 mg/L时效果最好,不但可以完全抑制农杆菌生长,而且有较高的芽诱导率。 |
| 关 键 词： | 花生 子叶 遗传转化 |
| 文章编号： | 1002-4093（2007）01-0013-07 |
| 收稿时间： | 2006-12-06 |
| 修稿时间： | 2006年12月6日 |
Research of Influence Factors on Peanut Cotyledon Regeneration in Genetic Transformation |
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| LIU Feng,SHAN Shi-hua,YAN Cai-xia,BI Yu-ping,LI Guang-min,LUN Wei-zhi.Research of Influence Factors on Peanut Cotyledon Regeneration in Genetic Transformation[J].Journal of Peanut Science,2007,36(1):13-19. | |
| Authors: | LIU Feng SHAN Shi-hua YAN Cai-xia BI Yu-ping LI Guang-min LUN Wei-zhi |
| Institution: | 1. College of Life Science, Shandong Normal Uni., Jinan 250014, China; 2. Shandong Peanut Research Institute, Qingdao 266100, China; 3. High-Tech Research Center, Shandong Academy of Agricultural Sciences, Jinan 250100, China; 4. Lingyang Agricultural Technique Station of Juxian, Juxian 276500 ; 5. Chengyang Agricultural Technique Station of Juxian, Juxian 276500 |
| Abstract: | Two peanut cultivars were used as source of cotyledon explants in this experiment.The percentage of shoots induced reached 89.67% and 83.33% respectively in MSC1 with 5.00 mg/L 6-BA and 1.50 mg/L 2,4-D.Transformation efficiency could be affected by many factors in the system of cotyledon explants.The better transformation efficiency was obtained by using Agrobacterium tumefaciens strain LBA4404 with OD600 as 0.5~0.7 and with 10 min infection,3d co-cultivation and the proper concentration of wounded tobacco leaf extract(1.0 mL/50 mL).Media with 250 mg/L Cef and 250~500 mg/L Carb could not only lower pollution ratio but also get higher transformation efficiency. |
| Keywords: | peanut cotyledon genetic transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |