大花蕙兰原生质体分离的影响因素研究

本文以大花蕙兰为材料，采用正交试验的方法进行了取材部位，纤维素酶浓度，甘露醇浓度以及酶液各成分的浓度等不同因素对其原生质体分离影响的研究。结果表明，以叶片为材料，在１％纤维素酶＋０．３％果胶酶＋０．２％半纤维素酶＋１１％甘露醇的组合中酶解，经２５±１℃，３０ｒ／ｍｉｎ，筛网过滤，离心洗涤，漂浮纯化，获得的原生质体产量最高，可达到１０￣６个鲜重。

Study on Protoplasts Isolation From Cymbidium hybridum

By orthogonal design, this paper studied the factors affecting the yield of proto-plasts isolation from Cymbidium hybridum.The highest yield,as high as 106/g FW ,was ob-tained from lysis of leaf tissue in cpw -11M solution containing 1 % cellulase,0.3% pectinase,0.2% hemicellulase,and 11 % mannitol.