两株广西伪狂犬病病毒的分离鉴定

从广西不同地方的发病仔猪脑内分离到两株病毒，分别命名为Ｂ株和Ｗ株。用这两株病毒分别接种家兔，产生的症状和病变与伪狂犬病病毒强毒ＭｉｎＡ株相似。将两株病毒在乳兔肾细胞和ＣＥＲ细胞上连续传代，均稳定地出现与ＭｉｎＡ株相同的细胞病变（ＣＰＥ）。在ＣＥＲ细胞上进行微量血清和试验，ＭｉｎＡ株的抗血清能完全中和Ｂ株和Ｗ株对细胞的致病变作用，表明两个毒株与ＰｒＶ强毒具有相似的抗原性。电镜下观察Ｂ株和Ｗ株病毒粒子

Isolation and identification of two isolates of pseudorabies virus in Guangxi

Two field strains of Pseudorabies Virus (PrV) were isolated from brains of dead piglets of different farms in Guangxi,Which were named B and W strains respectively.Rabbits inoculated with B and W strains showed typical syndrome of pseudorabies disease .Both B and W strains could cause the same CPE steadily as MinA strain,when they were passaged serially on PK cells or CER cells.Shape and structure were similar with PrV MinA strain when observed under elctron mi croscope.The results of microserum neutralization test showed that B and W strains had the same antigenicity as PrV MinA strain .In order to effectively distinguish the vaccine and field isolates of PrV,two pairs of primers based on sequences of PrV gp 50 and gI gens have been synthetized.The PG 1/PG 2 primers(from gp 50 ) amplified a fragment with desired size of 217 bp from standard PrV MinA strain,two isolates and vaccine of PrV.Amplification using the primers PI 1/PI 2(from gI) was also subjected to the four strains. However only MinA strain and two field isolates were positive,amplifying a fragment with desired size of 526 bp. PI 1/PI 2 could not amplify any fragment from vac cine strain that a gI gene was deleted.Results of this study proved that the two isolates belonged to virulent virus of PrV.