高效菌株的筛选及其抑菌物质的初分析

通过8个菌株对物树烂皮病病原菌菌丝及孢子萌发抑制作用的比较,筛选得到的高效菌株有：鳞柄白鹅膏（Amanita virosa）、珊瑚菌（Ramaria ephemeroderma）、白霜杯伞（Clitocybe dealbata）。对3种菌株粗提物进行紫外可见光谱扫描的结果显示：珊瑚菌活性物质粗提物的发色团吸收峰分布在250-275 nm、280-300 nm、360 nm;白霜杯伞活性物质粗提物的发色团吸收峰分布在250-300 nm、360 nm左右;鳞柄白鹅膏粗提物的发色团吸收峰分布在260 nm、275 nm和360 nm;紫外光谱分析的结果为抑菌活性物质的提取、制备和结构鉴定提供了有益的参考。高效菌株液体摇床培养粗提物对绿豆芽的生长及蛋白质的合成都有不同程度的抑制作用,其中鳞柄白鹅膏抑制作用最为明显。化感作用的研究结果为抑菌活性菌株应用于开发新型生物生长调节剂和新型的生物杀菌剂提供了理论依据。

Selected Highly-effect Strains and Preliminary analysis the Inhibition Substances

Through compared the inhibition efffect about 9 strains on pathogenic fungi mycelia and spores,Amanita virosa,Ramaria ephemeroderma and Clitocybe dealbata were selected as highly-effect strains.Then Uv-Vis spectra scan of crude extracts of 3 strains were done.The results showed that Ramaria ephemeroderma had absorption peaks respectively at 250-275 nm,280-300 nm and 360 nm;Clitocybe dealbata had absorption peaks respectively at 250-300 nm、360 nm;Amanita virosa had absorption peaks respectively at 260 nm、275 nm and 360 nm.The studies of spectrum scan would provide the beneficial reference for extraction and structure identification of inhibition activity substance.These highly-effect strains had inhibition effect on mung bean sprout grow and protein synthesis,in which the inhibition effect of Amanita virosa were highest.The studies results of the allelopathy would provide the theoretical basis for applying inhibition activity to develop new biological growth regulator or new biological fungicide and the allelopathy.