| 罹病光肩星天牛幼虫分离菌株的16 S rDNA系统发育分析 |
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| 引用本文： | 邓彩萍,刘红霞,闫喜中,武旭霞,骆有庆.罹病光肩星天牛幼虫分离菌株的16 S rDNA系统发育分析[J].农业科学与技术,2008,9(2). |
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| 作者姓名： | 邓彩萍 刘红霞 闫喜中 武旭霞 骆有庆 |
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| 作者单位： | [1]北京林业大学省部共建森林培育与保护教育部重点实验室,北京100083 [2]山西农业大学农学院,山西太谷030801 [3]呼和浩特职业学院生物应用工程系,内蒙古呼和浩特010051 |
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| 基金项目： | 北京市教委科研基地共建项目，教育部高等学校博士学科点专项科研基金，教育部长江学者和创新团队发展计划 |
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| 摘 要： | BH-1是在北京大必区一片旱柳树林的光肩星天牛刻槽中分离所得的细菌，室内杀虫生测试验表明其对天牛低龄幼虫有显著的致死作用。笔者通过16S rDNA序列分析和生化指标测定，对BH-1菌株进行丁鉴定。
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| 关 键 词： | 光肩星天牛 幼虫 16S rDNA序列分析 系统发育 |
Phylogenetic Analysis of the 16S rDNA of a Strain Isolated from Diseased Larva of Anoplophora glabripennis (Motsch.) |
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| DENG Cai-ping,LIU Hong-xia,YAN Xi-zhong,WU Xu-xia,LUO You-qing.Phylogenetic Analysis of the 16S rDNA of a Strain Isolated from Diseased Larva of Anoplophora glabripennis (Motsch.)[J].Agricultural Science ＆ Technology,2008,9(2). |
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| Authors: | DENG Cai-ping LIU Hong-xia YAN Xi-zhong WU Xu-xia LUO You-qing |
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| Abstract: | Objective] Study on the phylogenetic analysis of the 16S rDNA and insecticidal characteristics of strain BH-1 isolated from diseased larva of Anoplophora glabripennis (Motsch.) Method] The strain was identified by routine method and inoculated onto healthy Anoplophora glabripennis (Motsch.) for observing insecticidal effect, further 16S DNA was amplified by the specific primers for sequencing and homology analysis. Result] The mortality of second instar of Anoplophora glabripennis(Motsch.) reached 72.7% 8 d after 1010 cfu/ml BH-1 was inoculated. The homology of 16S DNA se- quences between BH-1 and Serratia marcescens accessed in GenBank reached 99.5%. Combined with the results of routine identification, BH-1 was identi-fied as S. marcescens. Conclusion] BH-1 could be used for biological control ofAnoplophora glabripenais (Motsch.). |
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| Keywords: | Anoplophora glabripennis (Motsch ) Serratia marcescens 16S rDNA Phylogeny |
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