家蚕副肌球蛋白/小副肌球蛋白基因的克隆及进化分析

[目的]克隆家蚕副肌球蛋白／小副肌球蛋白基因，分析该基因与运动性状的关系。[方法]利用PCR扩增和RACE技术获得了家蚕（Bombyx mori）副肌球蛋白的全长cDNA和小副肌球蛋白的部分cDNA；经检索家蚕wgs（基因组散弹序列）数据，获得了家蚕副肌球蛋白／小副肌球蛋白的基因组序列，并确定其基因组结构。[结果]家蚕PM/mPM基因由17个外显子和16个内含子组成，通过基因内部选择性启动子的使用，该基因组序列同时编码副肌球蛋白和小副肌球蛋白，小副肌球蛋白和副肌球蛋白共用最后6个外显子。家蚕副肌球蛋白与其他无脊椎动物副肌球蛋白的聚类分析表明，家蚕和野桑蚕有最近的亲缘关系，在昆虫纲中与黑腹果蝇关系最远。[结论]家蚕和野桑蚕副肌球蛋白的氨基酸序列间不存在可引起飞行缺陷的点突变，推测家蚕和野桑蚕飞行能力差异存在其他调控机制。

cDNA Cloning, Genomic Structure of PM/mPM Gene from B. mori

[Objective] The experiment aimed to clone paramyosin(PM) and mini-paramyosin(mPM) genes to analyze the relations between them and moving behaviors.[Method] PCR method and RACE technology were used to obtain whole cDNA of PM and some cDNA of mPM of Bombyx mori.By compared wgs of Bombyx mori,the genomic sequences of PM and mPM of Bombyx mori were obtained,besides,their genome structure was determined.[Result] PM and mPM genes were consisted of 17 exons and 16 introns.By the use of selective promoters,the gene sequence encoded PM and mPM,while PM and mPM shared the last 6 exons.The cluster analysis between PM of Bombyx mori and PM of other invertebrates demonstrated that the relation between Bombyx mori and Bombyx mandarina was closest and the relation between Bombyx mori and Drosophila melanogaster was farthest in Insecta.[Conclusion] There was no point mutation which could influenced flight in amino acid sequence of PM of Bombyx mori and Bombyx mandarina,so the difference of flight capacity of Bombyx mori and Bombyx mandarina might be regulated by other mechanism.