| 化学激活对小鼠卵母细胞孤雌发育的影响 |
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| 引用本文： | 岳福杰,董雅娟,柏学进,淡新刚,王雪红,赵兴,于进亮,张廷龙. 化学激活对小鼠卵母细胞孤雌发育的影响[J]. 安徽农业科学, 2006, 34(16): 4008-4009,4037 |
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| 作者姓名： | 岳福杰 董雅娟 柏学进 淡新刚 王雪红 赵兴 于进亮 张廷龙 |
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| 作者单位： | 莱阳农学院动物胚胎工程中心,山东,青岛,266109;莱阳农学院动物胚胎工程中心,山东,青岛,266109;西北农林科技大学家畜生殖内分泌与胚胎工程重点开放实验室,陕西,杨凌,712100 |
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| 摘 要： | 分别用不同浓度的氯化锶联合细胞松弛素、钙离子载体A23187和乙醇分别与6-二甲氨基嘌呤和细胞松弛素B刺激小鼠卵母细胞,比较卵母细胞的激活率和体外发育情况。结果表明,乙醇+6-DMAP+CB和A23187+6-DMAP+CB激活获得的激活率、桑囊胚率较高。
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| 关 键 词： | 小鼠 卵母细胞 孤雌激活 化学激活剂 |
| 文章编号： | 0517-6611（2006）16-4008-02 |
| 收稿时间： | 2006-04-21 |
| 修稿时间： | 2006-04-21 |
Effects of Chemical Activation on the Parthenogenetic Development of Mouse Oocytes |
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| YUE Fu-jie et al. Effects of Chemical Activation on the Parthenogenetic Development of Mouse Oocytes[J]. Journal of Anhui Agricultural Sciences, 2006, 34(16): 4008-4009,4037 |
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| Authors: | YUE Fu-jie et al |
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| Affiliation: | The Animal Embryo Engineering Center,Laiyang Agricultural College ,Qingdao,Shandong 266109 |
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| Abstract: | The objective of present study was to compare parthenogenic development of mouse oocytes through the treatment of the different medical methods with different concentration SrCl2 and 5 μmol/L cytochalasin B(CB)and either 10 % alcohol or 5 μmol/L calciumion A23187, and either 2 mM 6-dimethylaminopurine (6-DMAP) or 6-DMAP+5 μmol/L cytochalasin B(CB).In experiment, Oocytes were activated with 1.5, 5.0, 10.0 and 15.0 mmol/L SrCl2 and then incubated with 5 μnol/L CB. The results showed that Alchol-6-DMAP+CB had a higher rate of morula and blastocyst rates and activation rates than alchol-6-DMAP and alchol- CB(P<0.05), A 23187-6-DMAP +CB was also higher than A 23187-6-DMAPand A 23187-CB (P<0.05=. |
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| Keywords: | Mouse Oocytes Parthenogenesis Chemical activation reagent |
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