| 酶法提取虎杖中白藜芦醇的工艺优化 |
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| 引用本文： | 王林,何宗琴,郑在,尹若春. 酶法提取虎杖中白藜芦醇的工艺优化[J]. 安徽农业科学, 2013, 41(8): 3339-3340,3381 |
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| 作者姓名： | 王林 何宗琴 郑在 尹若春 |
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| 作者单位： | 安徽大学生命科学学院,安徽合肥,230601;安徽大学生命科学学院,安徽合肥,230601;安徽大学生命科学学院,安徽合肥,230601;安徽大学生命科学学院,安徽合肥,230601 |
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| 基金项目： | 国家自然科学基金，亚热带农业生物资源保护与利用国家重点实验室开放课题，大学生创新创业训练计划项目 |
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| 摘 要： | [目的]对酶法提取虎杖中白藜芦醇的工艺条件进行优化。[方法]采用单因素试验与正交试验相结合的方法,确定用酶法从虎杖中提取白藜芦醇的最佳工艺条件。[结果]从虎杖中提取白藜芦醇的最佳工艺为:料水比1∶15(V/V),漆酶含量0.30%,纤维素酶含量0.50%,木聚糖酶含量0.40%,β-葡聚糖酶含量0.20%,pH 4.4,在此条件下白藜芦醇的得率达0.592%。[结论]该研究为白藜芦醇的开发及综合利用提供了研究基础。
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| 关 键 词： | 虎杖 白藜芦醇 酶 |
Optimization of Extraction Process of Resveratrol from Polygoni cuspidati by Enzymatic Method |
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| Affiliation: | WANG Lin et al(College of Life Science,Anhui University,Hefei,Anhui 230301) |
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| Abstract: | [Objective] The paper was to optimize the extraction process of resveratrol from Polygoni cuspidati by enzymatic method.[Method] The best conditions of extraction of resveratrol from P.cuspidati by enzymatic method were determined by using the method of single factor experiment combined with orthogonal experiment.[Result] The best extraction process of resveratrol from P.cuspidati was that,the ratio of material and water was 1∶15(V/V);laccase content was 0.30%;cellulose content was 0.50%;xylanase content was 0.40%;β-glucanase content was 0.20%;pH 4.4,and the yield of resveratrol under these conditions was up to 0.592%.[Conclusion] The study provided a research basis for the development and utilization of resveratrol. |
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| Keywords: | Polygoni cuspidati Resveratrol Enzyme |
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