羊肉中8种有机磷农药残留的测定及基质效应

 【目的】建立气相色谱法同时测定羊肉组织中敌百虫等8种有机磷农药残留量，并对检测中的基质效应现象进行研究。【方法】样品用乙腈提取，N-丙基乙二胺作固相分散净化剂，火焰光度检测，基质匹配标准溶液内标法进行校准。【结果】羊肉组织中敌敌畏、乐果和马拉硫磷基质效应明显，其它几种有机磷农药基质效应较弱。建立的方法在2.5～300 μg?kg-1，线性良好，在50、100和200 μg?kg-1添加水平上，8种有机磷农药回收率为70.0%～103%，变异系数＜20%，检测限为0.5～1.9 μg?kg-1。【结论】建立的方法前处理简单、灵敏、准确、可靠，可以作为羊肉等动物性食品中有机磷农药残留量的检测。

Determination of 8 Organophosphorous Pesticide Residues in Mutton and Matrix Effect

【Objective】 A gas chromatographic method was established for determination of eight organophosphorous pesticides residues in mutton and analysis of matrix effects. 【Method】 The residues of organophosphorous pesticides in mutton were extracted by using acetonitrile and then the extracts were cleaned up using primary secondary amine as dispersive-solid-phase extract agent. The internal method of matrix-matched calibration standard was used for quantification. 【Result】 The matrix effect of dichlorvos, malathion and dimethoate was obvious, however, that of other organophosphorous pesticides was not obvious in mutton. The linearity was good in the range of 2.5 and 300 μg?kg-1 and the average recoveries of the organophosphorous pesticides from mutton fortified at 50, 100 and 200 μg?kg-1 were between 70.0% and 103%, with relative standard deviations of less than 20%. The limits of detection were about 0.5-1.9 μg?kg-1 for eight organophosphorous pesticides in mutton. 【Conclusion】 A simple, sensitive and reproducible method was developed and could be used to determine the residues of organophosphorous pesticides in mutton or other animal tissues.