| 燕麦DNA在小麦抗条锈病育种中的利用 |
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| 引用本文： | 刘萍,张立杰,刘生祥,马宏玮,徐兆桢. 燕麦DNA在小麦抗条锈病育种中的利用[J]. 植物保护学报, 2002, 29(4): 305-308 |
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| 作者姓名： | 刘萍 张立杰 刘生祥 马宏玮 徐兆桢 |
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| 作者单位： | 宁夏大学农学院农学系,永宁,750105 |
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| 基金项目： | 宁夏回族自治区科委攻关项目(980012) |
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| 摘 要： | 以健壮燕麦Avena sativa L.为供体,以普通小麦品种宁春4号为受体,利用花粉管通道将燕麦总DNA导入小麦中。对供体、受体、导入后代各变异株系进行连续4年的成株期混合菌种的抗病性鉴定和2年的分生理小种鉴定。结果显示:供体对小麦条锈菌免疫,受体对小麦条锈菌各生理小种严重感染,导入后代中有6个株系对小麦条锈菌各生理小种均表现高抗。表明燕麦DNA上可能携有抗小麦条锈菌的新基因,并已通过花粉管通道进入小麦,整合到小麦的染色体组中,稳定地表达。
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| 关 键 词： | 燕麦 外源DNA导入 条锈病 抗病育种 |
| 收稿时间： | 2001-04-23 |
| 修稿时间： | 2001-04-23 |
Utilization of oat (Avena sativa L.) DNA in wheat breeding for resistance to yellow rust |
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| Liu Ping,Zhang Lijie,Liu Shengxiang,Ma Hongwei and Xu Zhaozhen. Utilization of oat (Avena sativa L.) DNA in wheat breeding for resistance to yellow rust[J]. Acta Phytophylacica Sinica, 2002, 29(4): 305-308 |
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| Authors: | Liu Ping Zhang Lijie Liu Shengxiang Ma Hongwei Xu Zhaozhen |
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| Affiliation: | Agronomy Department, Ningxia University, Yongning 750105, China,Agronomy Department, Ningxia University, Yongning 750105, China,Agronomy Department, Ningxia University, Yongning 750105, China,Agronomy Department, Ningxia University, Yongning 750105, China and Agronomy Department, Ningxia University, Yongning 750105, China |
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| Abstract: | By taking oat (Avena sativa L. ) and common wheat (Ningchun No.4) as donor and recipient, respectively, total DNA prepared from oat was introduced into Ningchun No.4 by pollen tube pathway. The resistance of donor, recipient and all progenies to yellow rust (Puccinia stri-iformis ) were identified by inoculating mixed races with different virulence at adult stage during 19% - 1999. The results showed that the donor was immune, whereas the recipient was seriously susceptible . Six of the progenies were highly resistant to five physiological races of yellow rust. The result also indicated that the new genes resistant to yellow rust may be carried on oat DNA, which have been integrated into the genome of wheat and expressed steadily. |
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| Keywords: | Avena sativa L. exogenous DNA introduction Puccinia striiformis resistance breeding |
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