| 麝致病性大肠杆菌的质粒DNA分析 |
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| 引用本文： | 罗燕,程建国,郑士华,赵翠,李蓓,李敏.麝致病性大肠杆菌的质粒DNA分析[J].农业科学与技术,2009,10(3):22-25. |
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| 作者姓名： | 罗燕 程建国 郑士华 赵翠 李蓓 李敏 |
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| 作者单位： | 罗燕,郑士华,赵翠,李蓓,李敏,LUO Yan,ZHENG Shi-hua,ZHAO Cui,LI Bei,LI Min(四川农业大学都江堰分校生物科学系,四川都江堰,611830);程建国,CHENG Jian-guo(四川养麝研究所,四川都江堰,611830) |
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| 基金项目： | 四川省教育厅青年基金，四川农业大学青年科技创新基金 |
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| 摘 要： | 1材料与方法1．1菌株从四川养麝研究所白沙养麝场和金凤山养麝场病麝和死亡麝的化脓灶及脓汁、实质器官分离鉴定的24株麝致病性大肠杆菌菌株，其中Ecoli-1、Ecoli-7、E．coli-10、Ecoli-12、Ecoli-15、E．coli-16、Ecoli．17、E．coli-18、E．coli-23从金凤山养麝场分离，其余15株从白沙养麝场分离，均由四川农业大学都江堰分校微生物实验室保存。
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| 关 键 词： | 麝 致病性 大肠杆菌 质粒DNA 分析 |
Plasmid DNA Analysis of Pathogenic Escherichia coli in Musk Deer |
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| LUO Yan,CHENG Jian-guo,ZHENG Shi-hua,ZHAO Cui,LI Bei,LI Min.Plasmid DNA Analysis of Pathogenic Escherichia coli in Musk Deer[J].Agricultural Science ＆ Technology,2009,10(3):22-25. |
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| Authors: | LUO Yan CHENG Jian-guo ZHENG Shi-hua ZHAO Cui LI Bei LI Min |
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| Institution: | 1. Department of Biological Sciences, Sichuan Agricultural University Dujiangyan Campus, Dujiangyan 611830; 2. Sichuan Institute of Musk Deer Breeding, Dujiangyan 611830) |
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| Abstract: | Objective] The pathogenic Escherichia coli in musk deer was classified at molecular level to provide basic materials for molecular epidemiology of pathogenic Escherichia coli in musk deer. Method] Plasmids from 24 pathogenic Escherichia coli in musk deer were extracted by the Lysis Triton method, and then identified by single enzyme digestion with three endonucleases of Hind Ⅲ, EcoR Ⅰ and BamH Ⅰ. Result] The yield rate of plasmids was 91.6%, and 24 pathogenic Escherichia coli in musk deer had the identical or similar plasmid profiles. Conclusion] Plasmid DNA analysis offers scientific basis for molecular epidemiology of pathogenic Escherichia coli in musk deer in Sichuan Institute of Musk Deer Breeding. |
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| Keywords: | Musk deer Pathogenic Escherichina coli Plasmid DNA Plasmid profile |
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