泥糊菜提取液抑菌作用研究

[目的]探讨泥糊菜的消炎、抗菌机理。[方法]泥糊菜的叶片烘干粉碎后，用95％乙醇浸提。以枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌为供试菌种，测定最低抑菌浓度MIC。提取液分别经不同温度不同时间处理后测定提取液的热稳定性。[结果]泥糊菜提取液对3种供试菌种都有抑菌作用，其中对金黄色葡萄球菌抑制作用最强，对枯草芽孢杆菌抑制作用最弱。泥糊莱提取液对金黄色葡萄球菌、大肠杆菌、枯草芽孢杆菌的最低抑菌浓度分别为2．5％、3．7％、8．0％。泥糊菜提取液具有良好的热稳定性。糊莱提取液在不同温度处理后，MIC无变化。[结论]泥糊莱提取液对金黄色葡萄球菌的抑菌能力最强，并具有良好的热稳定性，

Study on Bacteriostasis of Hemistepta lyrata Bunge Extract

[Objective] The study was to explore the antiphlogistic and antibacterial mechanism of Hemistepta lyrata Bunge.[Method] After the leaf of H.lyrata was dried and shattered,and then extracted by 95% ethanol.With Bacillus subitilis,Staphylococcus aureus,Escherichia coli as tested strains,the minimal inhibitory concentration(MIC) was measured.After the extract was treated at different temperatures with different times,its thermal stability was measured.[Result] The extract of H.lyrata had a bacteriostasis on all 3 types of tested strains.The inhibition to S.aureus was the strongest and that to B.subitilis was the weakest.The MIC of H.lyrata extract to S.aureus,E.coli,B.subitilis was 2.5%,3.7% and 8.0%.resp.The H.lyrata extract had a good thermal stability.After H.lyrata extract was treated at different temperatures,the MIC had no change.[Conclusion] The H.lyrata extract had the strongest inhibition to S.aureus and had a good thermal stability.