Identification of a new family of tandem repeats in Triticeae genomes |
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Authors: | EA Salina EG Pestsova IG Adonina AV Vershinin |
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Institution: | (1) Siberian Department of the Russian Academy of Sciences, Institute of Cytology and Genetics, Lavrentiev Ave. 10, Novosibrsk, 630090, Russia |
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Abstract: | A new family of cereal tandem repeats was isolated, characterised and designated as spelt-1. The family of repeats comprises
about 2% of the Aegilops speltoides genome; however, its content differs considerably in the genomes of various Triticeae
species. Copy number of the constituent sequence, relative to Ae. speltoides, proved to be 40-60 times reduced in the genomes
of tetraploid wheats, 400-fold reduced in the genome of Triticum monococcum, and 1200-2400 times in the genomes of the other
19 Triticeae species studied. Drastic difference in the copy number and homology extent of the spelt-1 family sequences between
Ae. speltoides and other diploid species allows the utilisation of these sequences as species-specific telomeric markers for
Ae. speltoides, provided stringent hybridisation conditions apply. RFLP (restriction fragment length polymorphisms) analysis
of spelt-1 reveals polymorphism between the above species. This study of spelt-1 organisation in different Triticum species
provided further substantiation of the polyphyletic origin of the B genome of polyploid wheat.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | Aegilops speltoides genome-specific sequences tandem repeats Triticeae |
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