| 鸭肝炎病毒A66弱毒株在鸡胚成纤维细胞上的培养 |
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| 引用本文： | 张小飞,解启发,潘孝成,赵瑞红.鸭肝炎病毒A66弱毒株在鸡胚成纤维细胞上的培养[J].中国兽医学报,2000,20(1). |
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| 作者姓名： | 张小飞 解启发 潘孝成 赵瑞红 |
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| 作者单位： | 安徽省农业科学院,畜牧兽医研究所,安徽,合肥,230031 |
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| 基金项目： | “九五”农业部畜牧重点科研项目!(95牧 -0 2 -0 4 -
0 6) |
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| 摘 要： | 将鸡胚致弱的鸭肝炎病毒 ( DH V) A6 6 株接种于鸡胚成纤维细胞 ( CEF)进行细胞培养 ,通过对细胞培养物进行病毒滴度测定、鸡胚中和试验和电镜检查 ,证明了 DHV A6 6 毒株能够在 CEF上增殖。试验还表明 ,犊牛血清对 DH V增殖具有明显的抑制作用。此外 ,还根据细胞培养物病毒滴度测定结果绘制出了病毒在 CEF上的生长曲线。
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| 关 键 词： | 鸭肝炎病毒 弱毒株 鸡胚成纤维细胞 培养 |
Culture of the Attenuated A66 Strain of Duck Hepatitis Vi rus in Chicken Embryo Fibroblast |
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| ZHANG Xiao-fei,XIE Qi-fa,PAN Xiao-cheng,ZHAO Rui-hong.Culture of the Attenuated A66 Strain of Duck Hepatitis Vi rus in Chicken Embryo Fibroblast[J].Chinese Journal of Veterinary Science,2000,20(1). |
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| Authors: | ZHANG Xiao-fei XIE Qi-fa PAN Xiao-cheng ZHAO Rui-hong |
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| Abstract: | The preliminary observation on the attenuated A 66 strain of duck hepatitis virus(DHV) in a chicken embryo fibrobalst(CEF) culture system was reported. The virus titer assay, virus neutralization test in chicken embryo and electron microscopic examination of ultrathin section of the cell culture indicated that the A 66 strain of DHV could propagate in CEF. It was shown that the serum of new born calf apparently inhibited propagation of DHV. The growth curve of DHV in CEF culture was drawn according to the viral titer assay. |
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| Keywords: | duck hepatitis virus(DHV) attenuated A 66 strain chicken embryo fibroblast(CEF) culture |
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