| 发酵条件对蛋白核小球藻脂肪含量的影响 |
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| 引用本文： | 王步江,樊秀花,周娜.发酵条件对蛋白核小球藻脂肪含量的影响[J].天津农学院学报,2012,19(1):34-37. |
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| 作者姓名： | 王步江 樊秀花 周娜 |
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| 作者单位： | 1. 天津农学院食品科学系,天津,300384
2. 天津工业生物技术研究所,天津,300308 |
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| 基金项目： | 天津农学院科研基金项目“产油微生物的选育及发酵工艺条件研究”，国家重点基础研究发展计划(973计划)项目“微藻能源规模化制备的科学基础” |
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| 摘 要： | 利用单因素和正交试验法研究了蛋白核小球藻（Chlorella pyrenoidos aFACHB-29）培养基中的碳源、氮源、碳氮比、pH、温度等对其生物量和脂肪含量的影响。首先用单因素方法对5个相关影响因素的效应进行了评价，并筛选出了对生物量生长和脂肪含量有显著影响的3个因素：葡萄糖浓度、硝酸铵浓度和pH；再用正交试验法确定了生物量生长的最佳条件为：葡萄糖浓度为2％，pH值为7．5，硝酸铵浓度为0．016％。最终，发酵液中的藻体含量可达到5．6g，L，脂肪含量可达到1．46g/L。
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| 关 键 词： | 小球藻 发酵条件 脂肪 生物柴油 |
Effect of Fermentation Condition on Lipid Concentration of ChloreUa pyrenoidosa |
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| WANG Bu-jiang,FAN Xiu-hua,ZHOU Na.Effect of Fermentation Condition on Lipid Concentration of ChloreUa pyrenoidosa[J].Journal of Tianjin Agricultural College,2012,19(1):34-37. |
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| Authors: | WANG Bu-jiang FAN Xiu-hua ZHOU Na |
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| Institution: | 1.Department of Food Science,Tianjin Agricultural University,Tianjin 300384,China;2.Tianjin Institute of Industrial Biotechnology,Tianjin 300308,China) |
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| Abstract: | The effects of carbon source,nitrogen source,carbon-nitrogen ration,pH value and temperature on the biomass and lipid concentration were studied in the fermentation condition of Chlorella pyrenoidosa FACHB-29 by the single factor and orthogonal experiments.First,the single factor was used to evaluate the influence of the related factors.Then,the three factors affecting the biomass and lipid concentration notably were screened out as glucose concentration,ammonium nitrate concentration and pH value.Finally,the optimum conditions for the cell growth were determined through the orthogonal test,that is,2%glucose concentration,0.016%ammonium nitrate concentration,and pH value 7.5.Under the optimum conditions,the Chlorella pyrenoidosa cell concentration reached 5.6 g/L in the fermentation,and the lipid yield reached 1.46 g/L. |
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| Keywords: | Chlorellapyrenoidosa fermentation condition lipid biodiesel |
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