| 菩提树组织培养技术研究 |
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| 引用本文： | 王雅稚,孙宁,张磊,陈小强,许燕萍. 菩提树组织培养技术研究[J]. 天津农学院学报, 2012, 19(2): 30-33 |
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| 作者姓名： | 王雅稚 孙宁 张磊 陈小强 许燕萍 |
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| 作者单位： | 1. 天津农学院农学系,天津,300384
2. 天津滨海中新生态农业发展有限公司,天津,300480 |
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| 基金项目： | 天津市科技支撑计划项目“非洲紫罗兰新品种的引进及培育技术的研究”，天津市滨海新区科技计划项目“名贵花卉、苗木新品种引进、快繁及设施栽培技术的研究”，天津农学院大学生科技创新活动项目“菩提树组织培养无性快速繁殖技术的研究” |
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| 摘 要： | 以菩提树的带芽茎段为外植体，研究了不同外植体消毒时间及外源激素水平对菩提树组织培养的影响。试验结果表明，外植体以0．1％的HgCl2消毒10min可获得较低的污染率和较高的成活率；初代培养与分化增殖均采用MS为基本培养基，适宜的激素组合分别为NAA0．1mg/L＋6-BA1,0mg／L、NAA0．3mg/L＋6-BA1．O～2．0mg／L；试管苗生根培养以1／2MS＋NAA0．5mg／L为最佳，生根率达92．5％，植株生长健壮，适于移栽。污染的试管苗在移栽前进行灭菌药剂浸根处理，也可达到较高的成活率，可避免种苗的浪费。
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| 关 键 词： | 菩提树 茎段 组织培养 |
Tissue Culture of Ficus religiosa |
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| WANG Ya-zhi,SUN Ning,Corresponding Author,ZHANG Lei,CHEN Xiao-qiang,XU Yan-ping. Tissue Culture of Ficus religiosa[J]. Journal of Tianjin Agricultural University, 2012, 19(2): 30-33 |
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| Authors: | WANG Ya-zhi SUN Ning Corresponding Author ZHANG Lei CHEN Xiao-qiang XU Yan-ping |
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| Affiliation: | 1.Department of Agronomy,Tianjin Agricultural University,Tianjin 300384,China;2.Tanjin Binhai New Ecological Agriculture Development CO.,LTD,Tianjin 300480,China) |
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| Abstract: | Selecting the stem segment with buds as explants,the tissue culture technologies of Ficus religiosa were studied in the experiment.The results show that the optimum doused time was 8 to 10 min in 0.1% HgCl2,MS+NAA 0.1 mg/L+6-BA 1.0 mg/L was the best medium for inducing axillary buds,the proliferation coefficient was 4.5 on the medium of MS+NAA 0.3 mg/L+6-BA 1.0～2.0 mg/L,the suitable medium for rooting was 1/2 MS+NAA 0.5 mg/L,and the rate of rooting was 92.5%.The higher survival rate could be obtained,with the roots of contaminative plantlets being immersed in sterilized pharmaceutics before transplantation. |
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| Keywords: | Ficus religiosa stem segment tissue culture |
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