T0901317 induces apoptosis of human breast cancer MDA-MB-231 cells by up-regulating LXRα expression |
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Authors: | TU Jian LU Kai-qiang DING Wei-ke LIU Xiao-wang XIONG Ting LIU Ling YAN Xin ZHOU Zhi-gang |
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Affiliation: | 1. Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421001, China;2. The First Affiliated Hospital, University of South China, Hengyang 421001, China |
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Abstract: | AIM: To investigate the pro-apoptotic effect of T0901317, an artificial agonist of liver X receptor α (LXRα), on human breast cancer MDA-MB-231 cells and its mechanism. METHODS: MDA-MB-231 cells were treated with different concentrations (0, 10, 20 and 40 μmol/L) of T0901317 for different time (0, 12, 24 and 48 h). The cell apoptosis was determined by Annexin V/propidium iodide staining and Hoechst 33342 staining. The expression of apoptosis-related proteins, such as Bcl-2, caspase 3 and cleaved caspase-3, and LXRα was determined by Western blot. The mRNA expression of Bcl-2 and LXRα was analyzed by RT-qPCR. RESULTS: T0901317 induced the cell apoptosis in a dose-and time- dependent manner. The expression of cleaved caspase-3 and LXRα was up-regulated, but Bcl-2 was down-regulated by T0901317. The mRNA expression of Bcl-2 was down-regulated, while LXRα was up-regulated by T0901317.CONCLUSION: T0901317 up-regulates LXRα expression and induces the apoptosis of MDA-MB-231 cells. |
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Keywords: | T0901317 Liver X receptor α MDA-MB-231 cells Apoptosis |
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