Analysis of the CpG Methylation Pattern on GDF11 Gene Exon 1 in Ujumqin Sheep |
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| Authors: | HE Xiao-long LI Bei FU Shao-yin LIU Yong-bin QI Yun-xia WANG Biao TERI Gele RONG Wei-heng DA Lai |
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| Institution: | 1. Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, China;2. College of Animal Science, Inner Mongolia Agricultural University, Hohhot 010018, China |
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| Abstract: | In order to investigate the methylation patterns on growth differentiation factor 11 (GDF11) gene exon 1 in Ujumqin sheep,we compared the level of methylation of GDF11 gene exon 1 in common Ujumqin sheep (CUS) and multi-vertebrae Ujumqin sheep (MUS) using the bisulfite sequencing PCR (BSP) method.The results showed that the average methylation rate of GDF11 gene exon 1 for MUS was extremely significantly higher than that for CUS (P<0.01),the average methylation rate of common Ujumqin sheep and multi-vertebrae Ujumqin sheep were 0.123 and 0.569.Analysis of 13 CpGs of GDF11 gene exon 1 showed that the CpG_11 and CpG_13 methylation sites had the highest methylation rate with a combined methylation value of 90% for MUS.Our results suggested that differential DNA methylation of GDF11 gene exon 1,in particular at these two sites,might cause the increase in the number of vertebrae in Ujumqin sheep. |
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| Keywords: | Ujumqin sheep GDF11 gene CpG site methylation bisulfite sequencing |
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