Cloning of GABRP Gene and Its Expression in Buffalo Mammary Tissue |
| |
Authors: | HAO Wen-yan CHEN Pei-fang DAI Xiao-li WANG Ping WU Da-ping SHI De-shun LI Xiang-ping |
| |
Affiliation: | State Key Laboratory of Subtropical Bioresourse Conversation and Utilization, Guangxi University, Nanning 530004, China |
| |
Abstract: | GABA is an important inhibitory neurotransmitter in the brain,beside the function of transmissing information,which plays an important role in endocrine tissues.In this study,the CDS of buffalo GABRP gene was cloned and its biological information was analyzed,the expression of GABRP in buffalo mammary tissue was also examined by Real-time quantitative PCR and immunohistochemistry.The RT-PCR results showed that GABRP CDS fragment was obtained successfully from Guangxi local buffalo,which shared 99% with that of river buffalo,its length was 1 401 bp.The phylogenetic tree showed that GABRP had high conservation in different species.The immunohistochemistry results showed that buffalo GABRP protein located at the alveolar epithelial cells of buffalo mammary.The expression of GABRP gene in non-lactation buffalo mammary was significantly higher than that of lactation tissue (P<0.05).The results laid a foundation for further study the function of GABRP gene in the development of buffalo mammary gland. |
| |
Keywords: | buffalo GABRP gene mammary tissue |
|
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
|
点击此处可从《中国畜牧兽医》下载全文 |
|