Establishment and Optimization of INH Epitope Peptide Vaccine for Detection of Antibody |
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Authors: | ZHANG Ling-ling YUE Yao-jing FENG Rui-lin LI Hong-feng GUO Ting-ting YUAN Chao NIU Chun-e LIU Jian-bin SUN Xiao-ping HAN Ji-long LIU Shan-bo YANG Bo-hui |
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Affiliation: | 1. Lanzhou Institute of Animal Science & Veterinary Pharmaceutics, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China;2. Tianzhu County Animal Husbandry Technology Extending Stations, Tianzhu 733200, China |
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Abstract: | In this study, an indirect ELISA method was established to detect inhibin hormone (INH) epitope peptide vaccine antibody, it would provide oretical reference for the determination of Fine-wool sheep after active immune body INH epitope peptide vaccine antibody. On the basis of the predecessors, using indirect ELISA method to determinate serum INH epitope peptide antibody levels of sheep, and through control different experimental conditions to look for the best experimental conditions. Through explorating the experimental conditions, finally, the testing experiment conditions were determined, which was blocked solution with skimmed milk powder, INH and GnIH synthetic peptides dilution degrees for 20 000 times, the optimum reaction time was 60 min, the best color action time was 15 min. In this experiment, a kind of method to detection antibody in the body after INH active immune sheep was built, it would provide a reference for future research. |
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Keywords: | inhibin hormone Fine-wool sheep active immunization reproductive hormone |
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