重组减毒沙门氏菌ΔcrpC79-13(pcDNA3-HN)在雏鸡体内主要组织器官定植情况检测

为检测重组减毒沙门氏菌ΔcrpC79-13（pcDNA3-HN）在雏鸡体内主要组织器官定植情况，本研究将120只1日龄雏鸡随机分成3组，分别用减毒沙门氏菌ΔcrpC79-13、重组减毒沙门氏菌ΔcrpC79-13（pcDNA3-HN）和PBS免疫，在免疫后4、24、48、72、96、120、144及168 h，分别检测减毒沙门氏菌ΔcrpC79-13和重组减毒沙门氏菌ΔcrpC79-13（pcDNA3-HN）在雏鸡心脏、肝脏、脾脏、盲肠和血液的定植情况。结果显示，在免疫后24 h减毒沙门氏菌ΔcrpC79-13在血液中数量达到了一个峰值，24～48 h血液细菌数量下降，48～96 h血液中细菌数上升并在96 h达到另一个峰值，96～168 h血液中细菌数量下降，在心脏、肝脏、脾脏、盲肠这几个脏器中，细菌的数量分别在4～72 h呈上升趋势并在72 h达到峰值，72～96 h脏器细菌数量下降，96～120 h菌数上升并在120 h达到峰值，随后细菌数量下降；免疫重组减毒沙门氏菌ΔcrpC79-13（pcDNA3-HN）组在雏鸡各脏器细菌总数变化及定植情况与ΔcrpC79-13相似。结果表明，重组菌株ΔcrpC79-13（pcDNA3-HN）与亲本菌株ΔcrpC79-13均可在雏鸡心脏、肝脏、脾脏、盲肠等器官定植，且在各脏器中菌株定植变化趋势一致但数量下降。

Colonization Detection of Recombinant Attenuated Salmonella ΔcrpC79-13 (pcDNA3-HN) in Major Organs of Chicks

The present study was designed to detect ΔcrpC79-13(pcDNA3-HN) which was a recombinational attenuated Salmonella embedded status in chicks major organs,120 one-day chicks were randomly assigned into 3 treatments according to a single factorial arrangement,which were immunized by ΔcrpC79-13,recombinational ΔcrpC79-13(pcDNA3-HN) and PBS,respectively.After immunization for 4,24,48,72,96,120,144 and 168 h,the embedded status of ΔcrpC79-13and recombinational ΔcrpC79-13(pcDNA3-HN) was detected in the heart,liver,spleen,cecum and blood of chicks.The results showed that the amount of attenuated immune Salmonella ΔcrpC79-13 reached a peak in the blood within 24 h,blood bacterial population decline in 24 to 48 h, number of bacteria in the blood rose at 48 to 96 h and reached another peak in 96 h,and the bacteria of the blood fell at 96 to 168 h.In the heart,liver,spleen and cecum,the number of bacteria in these organs at 4 to 72 h had an upward trend and reached a peak at 72 h,viscera bacterial population declined at 72 to 96 h,and the count rose at 96 to 120 h and reached a peak at 120 h,then the number of bacteria decreased.It was similar to the change in each viscera strain of group ΔcrpC79-13(pcDNA3-HN).These data indicated that both of the recombinational bacterial strains ΔcrpC79-13(pcDNA3-HN) and parental bacterial strain ΔcrpC79-13 could be embedded in a series of organ of chicks such as heart,liver,spleen,and cecum.Meanwhile,there was a consistent declining trend of bacterial strain amount in each viscus of chicks.