Gene Structure Prediction and Molecular Evolution Analysis of Raccoon Dog agouti Gene based on RNA-Seqs |
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Authors: | ZHANG Hao ZHAO Jia-ping WU Qiong YANG Ying DU Zhan-yu GUO Yue-yue XING Xiu-mei XU Chao ZHANG Lin-bo |
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Institution: | 1. Jilin Agricultural University, Changchun 130118, China;
2. Key Laboratory of Special Economic Animal Genetic Breeding and Reproduction, Ministry of Agriculture, State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Science of CAAS, Changchun 130112, China |
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Abstract: | To in-depth analyze the molecular structure characteristics of agouti gene,using bioinformatic methods for RNA-Seqs test to get the complete coding sequence of raccoon dog agouti gene,and its coding protein structure was forecasted and analyzed.The results showed that the length of raccoon dog agouti gene cDNA was 530 bp which contained 396 bp open reading frame (ORF),encoded 131 amino acids.Predicting the weight of the raccoon dog agouti protein molecular was 14.41 ku and isoelectric point (pI) was 9.68,as the unstable hydrophohbic protein;It contained 11 phosphorylation sites,1 glycosylation sites and a long reach 24 amino acid signal peptide;Random coil was the main secondary structure.Phylogenetic tree showed that the raccoon dog had a close genetics distance to vuipe and canis familiaris which was consistent with the traditional animal taxonomy.Through the analysis of the molecular structure characteristics of raccoon dog agouti gene,to provide the oretical basis for revealing the influence of the colour diversity molecular genetics mechanism. |
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Keywords: | raccoon dog agouti gene agouti signaling protein(ASIP) bioinformatics |
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