C-reactive protein induces apoptosis in human renal tubular epithelial cells |
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Authors: | XIE Kai-qing YANG Hai-bo LIN Hong-sheng CHEN Li HUO Dong-mei SHI Ying-long QIN Shi-yan ZHOU Hong-wei |
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Affiliation: | 1. Renal Division of The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China;2. Department of Microbiology, Guangxi Medical University, Nanning 530021, China |
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Abstract: | ATM: To explore whether the C-reactive protein (CRP) level in microinflammation state induces the apoptosis of renal tubular epithelial cells. METHODS: HK-2 cells were stimulated with recombinant human CRP. Annexin-FITC-PI staining and flow cytometry were used to detect the percentage of apoptotic cells. Morphology observation of apoptosis was assessed by Hoechst 33258 staining. Caspase-3 activity was measured by a colorimetric assay. The expression of apoptotic gene bax and anti-apoptotic gene bcl-2 at mRNA levels was determined by real-time PCR. RESULTS: CRP induced apoptosis of HK-2 cells in a time- and dose-dependent manner. The maximal apoptotic effect of CRP concentration was 10 mg/L CRP at concentration of 20 mg/L. CRP treatment was associated with the characteristic morphological features of apoptosis such as condensation, fragmentation or margination of nuclear chromatin. CRP exposure increased caspase-3 activity, up-regulated the mRNA expression of Bax and down-regulated the mRNA expression of Bcl-2. CONCLUSION: Slightly increased CRP level has the potential to induce apoptosis of renal tubular cells. |
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Keywords: | C-reactive protein Renal tubular epithelial cells Apoptosis |
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