MSTN基因在1~6月龄哈萨克羔羊肌肉组织中表达量及其与生长指标相关性分析

试验旨在分析MSTN基因在1~6月龄哈萨克羔羊肌肉组织中表达量及其与各项生长指标的相关性,为哈萨克羊的良种选育提供科学依据.随机选取5只哈萨克公羔羊,每月定时在羔羊活体腿部取肌肉组织,软尺测量体长、体高、体重、管围、胸围等各项生长指标.实时荧光定量 PCR分析1~6月龄哈萨克羔羊肌肉组织中MSTN的表达量,相关性分析MSTN基因在羔羊肌肉中的表达量与各项生长指标的关联性.结果显示:哈萨克羔羊各项生长指标在1、2月龄快速增长,体重从4月龄开始增重缓慢,体长、体高、胸围在3月龄出现增长缓慢,管围在3月龄起基本停止增长.MSTN基因在1~4月龄哈萨克羔羊肌肉组织中表达量均无显著性差异(P>0.05),呈现略微上升的趋势,5月龄达到最高峰,6月龄迅速下降.MSTN 基因在肌肉中表达量与体长呈显著负相关 (P<0.05),相关系数为-0.472,与体重、胸围呈弱负相关,与管围、体高呈弱正相关.

Expression Levels of MSTN gene in Muscle Tissues and Its Association with Growth Indexes in 1-6 Months Old Kazak Lamb

The experiment was carried out to investigate the MSTN gene expression in muscle tissues and its association with growth indexes in 1-6 months old Kazak lamb,and provide genetic information for breeding Kazak lambs.Five Kazak lambs were randomly chosen and leg muscle samples were collected monthly.Body length,body height,body weight,pipe circumference and chest circumference were measured.MSTN gene relative expression levels in 1-6 months old Kazak lamb muscle tisses were determined using Real-time PCR and its association with growth indexes were analysised.The results showed that: Kazak lambs grew rapidly at the age of 1 and 2 months.From 4 months,body weight increased slowly and body length,body height and chest circumference rose slowly meanwhile pipe circumference stopped growing from 3 months.At 5 and 6 months,the growth indexes had not significant difference (P>0.05).MSTN gene expression had not significant difference in 1-4 months old Kazak lamb muscle tissues (P>0.05),showing a upward trendency.The expression was the highest at 5 months and lowest at 6 months.MSTN gene expression was significant negatively associated with body length (P<0.05) and the correlation coefficient was-0.472.And there was a weak negative association between MSTN gene expression with body weight and chest circumference,a weak positive correlation with body height and pipe circumference.