Expression of MMP-2 and NF-κB in myocardium of mice with viral myocarditis |
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Authors: | RUAN Miao-hua WANG Kai WANG Dan ZHOU Ai-hua CHU Mao-ping CHEN Qi QIAN Yan |
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Institution: | 1. Department of Pediatrics, The First Affiliated Hospital, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325027, China;
2. Department of Pediatric Cardiology, The Second Affiliated Hospital, Wenzhou Medical University, Wenzhou 325027, China |
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Abstract: | AIM: To observe the effects of TNF-α/nuclear factor-κB(NF-κB)/matrix metalloproteinase-2(MMP-2) pathway on the expression of MMP-2 in the mice with viral myocarditis. METHODS: Six-week-old inbred male mice were randomly assigned to control and myocarditis group. The mice in myocarditis group and control group were intraperitoneally inoculated with 0.1 mL 10-5.69 TCID50/mL coxsackievirus B3 and vehicle (PBS), respectively. Ten mice were sacrificed at the 4th and 10th days after injection. The blood and heart specimens were harvested. The serum content of TNF-α was measured by ELISA. The myocardial levels of MMP-2, NF-κB p65 and IκBα were determined by Western blot. RESULTS: Compared with control group, the protein expression of MMP-2 and NF-κB p65 in the myocardium and the serum content of TNF-α were significantly increased in myocarditis group (P<0.05). The protein expression of IκBα was lower in myocarditis group than that in control group (P<0.05).CONCLUSION: TNF-α, NF-κB p65 and MMP-2 were higher in the mice with acute viral myocarditis. The increased expression of them might be involved in the pathogenesis of viral myocarditis. |
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Keywords: | Myocarditis Matrix metalloproteinase-2 Nuclear factor-κB |
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