适配体传感器结合便携式血糖仪定量检测赭曲霉毒素A的方法研究

目前大多数小分子毒素的定量检测方法仍需以实验室为基础或者定制设备,不能被公众广泛应用。研究开发了一种便携式适配体生物传感器,结合血糖仪用于赭曲霉毒素A(OTA)的定量检测。所开发的适配体生物传感器的线性范围是1×10-8~4×10-6mol/L,检出限6.7×10-9mol/L(2.69μg/kg);适配体生物传感器被成功地应用于婴幼儿米粉和羊草样品检测,回收率达84%~122%,表明所开发的适配体生物传感器为OTA的定量检测提供了一种新的方法,展现出良好的应用前景。

Method Research of Quantitative Detection for Ochratoxin A by Aptasensor Combining Portable Glucose Meter

Despite years of research, most current methods for quantitative detection of small molecular toxins still require laboratory-based or customized devices that are not widely available to the general public for quantitative analysis. A novel portable biosensor for sensitive and selective detection of ochratoxin A (OTA) was developed based on the specific recognition between aptamer and OTA using a personal glucose meter (PGM) as readout. The enhanced signal of the PGM had a linear relationship with the concentration of OTA in the range of 1.0×10-8～4.0×10-6 mol/L, the detection limit was 6.7×10-9 mol/L (2.69 μg/kg). This method could be used to quantitatively detect OTA present in infant rice cereal and Chinese wildrye samples with good recoveries in the range of 84%～122%. This aptasensor provided a new method to determine OTA and displayed good potential for practical application.