Cloning and Sequence Analysis of Zfx/Zfy Gene in Cervus elaphus

Specific primers were designed according to the mRNA sequence of Bos taurus Zfx/Zfy genes provided in GenBank.Zfx/Zfy sequences of Cervus elaphus were cloned and analyzed.The results showed that the cloned Zfx gene sequence of Cervus elaphus was 2 115 bp (GenBank accession No:KP257294.1) that encoded 696 amino acids,the cloned Zfy gene sequence was 2 406 bp (GenBank accession No:KU041539) that encoded 801 amino acids.The phylogenetic trees of Zfx and Zfy amino acid were obtained using the Neighbor-Joining method of DNAStar software.Analysis showed that Zfx of Cervus elaphus was closely related to Homo sapiens,compared with Bison,Pantholops hodgosoniiand,Panthera tigris,Felis catus,Bos taurus,Canis lupus familiaris,Ovis aries.Zfy of Cervus elaphus was closely related to Bos taurus,Bison,Ovis aries,Pantholops hodgosonii compared with others.Zfx/Zfy genes of Cervus elaphus were cloned and sequenced for the first time,and this research provided a foundation for further studying the structure and biological functions of the proteins.