胡萝卜镰刀菌根腐病病原菌的鉴定

为明确内蒙古西部地区一种新病害——胡萝卜镰刀菌根腐病病原菌种类,采用组织分离法对病原菌进行分离,用胡萝卜片接种、有伤口和无伤口胡萝卜肉质根接种及盆栽接种进行致病性测定,并通过形态学和分子生物学方法对病原菌进行鉴定。结果表明,分离到的3个菌株F1、F2和F3均表现致病性。经形态学鉴定,F1菌株为尖孢镰刀菌(Fusarium oxysporum),F2和F3为茄病镰刀菌(Fusarium solani)。进一步采用真菌通用引物ITS1/ITS4对病原菌r DNA-ITS进行PCR扩增,并将测序结果在Gen Bank中进行同源性比对分析,鉴定结果和形态学鉴定的结果一致。明确了内蒙古西部地区胡萝卜镰刀菌根腐病的病原菌种类为尖孢镰刀菌(F.oxysporum)和茄病镰刀菌(F.solani)。

Identification of the Pathogen of Fusarium Causing Carrot Root Rot

In order to definit a new disease observed on carrot roots and its pathogen speciesof Fusarium causing carrot root rot，this paper isolated the pathogen with tissue isolation method，inoculated the carrot slices，and carried out pathogenicity identification with morphology andmolecular biology．Pathogenicity was tested with the inoculated carrot slices，inoculated woundedand non wounded carrots，and carrots planted in pots．The results indicated that 3 strains F1，F2 and F3 were obtained，and all isolates appeared typical symptoms and showed pathogenicity．The isolate F1 was identified as Fusarium oxysporum，the F2 and F3 were both identified as Fusarium solaniwith morphology preliminary．Sequences of ribosome rDNA-ITS of isolates were tested with primer ITS1/ITS4，the same results were obtained with morphology，which showed the pathogens were F. oxysporum and F. solani oncarrot root rot.