Hydrogen sulfide inhibits adenosine triphosphate-induced activation and IL-1β releases in rat microglia |
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Authors: | MA Jie WANG Jiao-jiao WANG Lu LI Xin-juan WANG Guo-hong ZHAO Hong-gang LI Dong-liang |
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Institution: | 1. Department of Neurology, The Central Hospital of Xinxiang, Xinxiang 453003, China;
2. Department of Physiology and Neurobiology, Xinxiang Medical University, Xinxiang 453003, China |
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Abstract: | AIM: To investigate the effects of sodium hydrosulfide (NaHS), a donor of hydrogen sulfide (H2S), on the membrane permeability, intracellular Ca2+ concentration (Ca2+]i) and the release of IL-1β induced by adenosine triphosphate (ATP) in rat microglia, and to explore the effect of H2S on ATP-P2X purinergic signaling pathway and the molecular mechanism of its neuroprotective effect. METHODS: Rat microglia in logarithmic growth phase were used in the study. TheCa2+]i was detected by Fura-2/AM staining. Fluorescent dye YO-PRO-1 was used to observe the membrane permeability. Interleukin-1β (IL-1β) was measured by rat IL-1β ELISA kits. RESULTS: The YO-PRO-1 fluorescence intensity was obviously elevated by ATP induction in a dose-dependent manner in the rat microglia, but this effect was counteracted by NaHS pretreatment (P<0.05).Ca2+]i rapidly increased and then decreased slowly, forming a stable platform for a long time when rat microglia were treated with ATP. Ca2+ spike activity induced by ATP had no change, but the platform disappeared (P<0.05) after NaHS pretreatment. The ATP and LPS together facilitated the release of IL-1β, but the phenomenon was inhibited by NaHS (P<0.05). CONCLUSION: Hydrogen sulfide may decrease the membrane permeability, calcium inflow and IL-1β release in rat microglia activated by high dose of ATP. The cytoprotection of hydrogen sulfide may be mediated by purinergic signaling pathway. |
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Keywords: | Adenosine triphosphate Purinergic P2X receptors Hydrogen sulfide Microglia |
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