Cloning and Bioinformatics Analysis of FoxN1 Gene of Min Pig

This study was intended to explore the structure and function of FoxN1 genes of Min pig.Min pig FoxN1 gene was cloned by PCR, the complete coding region of FoxN1 sequence was analyzed by bioinformatics methods, the FoxN1 gene sequences of Min pig and Large White pig were compared, and phylogenetic tree was built.The results showed that Min pig FoxN1 complete gene coding region was 1 941 bp, encoding 646 amino acids, molecular weight was 68.64 ku, theoretical isoelectric point was 5.81. FoxN1 protein located within the nucleus, it was not a secreted protein, in which signal peptide was not present and there was no transmembrane area.There was a FH domain in 269 to 347 amino acid position.The protein among different species was highly conserved.Three kinds of transcription splice variants were found.Compared with variant 1, there was deletion in the nucleotide position 124 to 387 in variant 2, and GCA insertion in 581 to 582 in variant 3.Compared with Large White pig, Min pig FoxN1 gene sequences existed four mutations, of which three were synonymous mutations, one was a missense mutation, resulting in the 108th amino acid changed.The phylogenetic tree revealed a close genetic relationship between Min pig and artiodactyla animals.The results showed the genetic structure of FoxN1 gene was complex, it would provide the basis for a further reveal on Min pig FoxN1 gene's function.