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Downregulated transient outward potassium channel protein Kv4.2 and Kv4.3 expression in PVN contributes to sympathoexcitation in rats with chronic heart failure
Authors:MA Liu-yi  YIN Yu-jie  WEI Geng  LI Hong-rong  ZHANG Jun-fang  LIU Huan  JIA Zhen-hua
Institution:1. Hebei Medical University, Shijiazhuang 050017, China; 2. Department of Cardiology, Yiling Medical Institute of Hebei Province, Key Laboratory of State Administration of Chinese Medicine(Cardio-cerebral Vascular Collateral Disease), Shijiazhuang 050035, China; 3. Department of Cardiology, Yiling Hospital of Hebei Medical University(Collateral Disease Theory Key Discipline of State Administration of Traditional Chinese Medicine), Shijiazhuang 050091, China
Abstract:AIM: To investigate the transient outward potassium channel protein expression in paraventricular nucleus(PVN) and its contribution to renal sympathetic nerve activity(RSNA) in rats with chronic heart failure(CHF).METHODS: A rat model of CHF was prepared by acute myocardial infarction that was induced by ligation of the left anterior descending coronary artery. Four weeks after heart failure, echocardiogram was applied to identify the CHF model and plasma norepinephrine(NE), serum NH2-terminal pro-brain natriuretic peptide(NT-proBNP) were detected by ELISA. The expression of ransient outward potassium channel proteins Kv4.2 and Kv4.3 at mRNA and protein levels was determined by real-time PCR and Western blot. The mean arterial pressure(MAP), heart rate(HR) and RSNA were measured in anesthetized rats with PVN microinjection of potassium channel blockers 4-AP. RESULTS: In CHF group, the rat cardiac function and Kv4.2 and Kv4.3 expression in PVN were obviously lower while plasma NE and serum NT-proBNP were obviously higher than those in sham group. Microinjection of 4-AP into PVN induced an increase in MAP, HR and RSNA in both sham and CHF rats, while the CHF rats exhibited smaller responses to 4-AP than sham-operated rats.CONCLUSION: Downregulation of Kv4.2 and Kv4.3 expression in the PVN may be a potential mechanism for sympathoexciation in the rats with chronic heart failure.
Keywords:Chronic heart failure  Sympathetic nervous  Hypothalamic paraventricular nucleus  Ransient outward potassium channel protein  
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