间歇浸没式生物反应器培养铁皮石斛组培苗

目前铁皮石斛种苗生产主要采用植物组织培养的方法,传统的固体培养方式成本高效率低,无法满足市场需求,利用间歇浸没式生物反应器可以减少人工投入,降低生产成本。利用自主研发的间歇浸没式生物反应器培养铁皮石斛种苗,同时以固体培养作对照,比较了两种培养方式的铁皮石斛增殖率及植株高度、茎粗、根系情况、移栽成活率等。结果显示间歇浸没式生物反应器培养获得的铁皮石斛增殖率更高,组培苗更健壮,而且根系适中,利于栽培,移栽成活率也高于固体培养,为铁皮石斛种苗生产提供了一种新的方法。

Seedlings Cultivation of Dendrobium offiicinale using Temporary Immersion Bioreactors

At present, Dendrobium officinale seedlings are mainly produced by plant tissue culture. Solid culture method is characterized by high production costs and low multiplication rates, which is unable to satisfy the increasing market demands. Temporary immersion bioreactors（TIB）can reduce labor and costs of production. This study compared the multiplication rates, plant height, diameter of stem, root system growth and survival rates after transplantation of D. officinale plantlets cultured in self-developed TIB and solid medium. The multiplication rates and quality of plantlets produced in TIB were significantly higher than those produced in solid medium. Plantlets produced in TIB with moderate root size were suitable for cultivation, and their survival rates after transplantation were significantly higher than those produced in solid medium. These results showed that TIB provided a new protocol for production of D. officinale seedlings.