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| 芥蓝花薹中莱菔硫烷含量的HPLC分析 | |
| 引用本文： | 李占省,刘玉梅,方智远,杨丽梅,庄,木,张扬勇,李凌云,孙培田.芥蓝花薹中莱菔硫烷含量的HPLC分析[J].中国蔬菜,2016,1(4):53. |
| 作者姓名： | 李占省 刘玉梅 方智远 杨丽梅 庄 木 张扬勇 李凌云 孙培田 |
| 作者单位： | 1.中国农业科学院蔬菜花卉研究所，北京 100081；;2. 农业部蔬菜品质监测检验中心，北京 100081 |
| 基金项目： | 国家自然科学基金项目（31372067，31501761），现代 农业产业技术体系建设专项（CARS-25-A），国家科技支撑计划 项目（2013BAD01B04），农业部园艺作物生物学与种质创制重点 实验室项目，中国农业科学院科技创新工程项目（CAAS-ASTIPIVFCAAS） |
| 摘 要： | 以23份芥蓝纯合自交系和21份杂交F_1为试材,采用反相高效液相色谱法(RP-HPLC)测定花薹中莱菔硫烷含量,分析芥蓝花薹中莱菔硫烷含量的变化规律。结果表明:44份芥蓝材料花薹中莱菔硫烷含量变异范围为46.89~419.45mg·kg~(-1)(DW),供试材料间莱菔硫烷含量差异达显著水平。获得了1份莱菔硫烷含量较高的F_1材料A86,含量为419.45mg·kg~(-1)(DW);3份莱菔硫烷含量较高的高代纯合自交系材料A1、A17和A53,含量分别为298.50、354.19、361.06mg·kg~(-1)(DW),可用于芥蓝抗癌新品种的选育和利用。 |
| 关 键 词： | 芥蓝 花薹 莱菔硫烷 高效液相色谱法 |
Analysis of Sulforaphane in Chinese kale（Brassica albograbra L. H. Bailey） by HPLC |
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| LI Zhan-sheng,LIU Yu-mei,FANG Zhi-yuan,YANG Li-mei,ZHUANG Mu,ZHANG Yangyong,LI Ling-yun,SUN Pei-tian.Analysis of Sulforaphane in Chinese kale（Brassica albograbra L. H. Bailey） by HPLC[J].China Vegetables,2016,1(4):53. | |
| Authors: | LI Zhan-sheng LIU Yu-mei FANG Zhi-yuan YANG Li-mei ZHUANG Mu ZHANG Yangyong LI Ling-yun SUN Pei-tian |
| Institution: | 1.Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；;2. Supervision; and Testing Center for Vegetable Quality，Ministry of Agriculture，Beijing 100081，China |
| Abstract: | Abstract：Forty four lines of Chinese kale（Brassica albograbra L．H．Bailey）including 23 high inbred lines and 21 F1 generations were used to detect the sulforaphane contents in flower stalk of Chinese kale by RPHPLC， and to analyze the changing rule in sulforaphane contents．The results showed that the changing range of sulforaphane contents in 44 Chinese kale was 46.89-419.45 mg·kg-1（DW），and there were significant differences between sulforaphane contents of the testing lines．Four lines were detected with higher sulforaphane contents，among them one line was F1 generation A86 with 419.45 mg·kg-1（DW）sulforaphane content，the other 3 were inbred lines A1，A17 and A53．Their sulforaphane contents were 298.50 mg·kg-1（DW），354.19 mg·kg-1（DW），361.06 mg·kg-1（DW），respectively．They could be used for breeding of Chinese kale in anti-cancer and marketing promotion． |
| Keywords: | Brassica albograbra L．H．Bailey Flower stalk Sulfoaphane High performance liquid chromatography（HPLC） |
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