Effects of PI3K/AKT signaling pathway on polarization of M2 macrophages induced by SjCystatin |
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Authors: | LIU Ju YANG Xiao YUE Yuan WANG Le-xun WU Zhong-kai |
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Institution: | Department of Cardiac Surgery II, The First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, China |
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Abstract: | AIM: To investigate the subtype of M2 macrophages induced by Schistosoma japonicum cystatin (SjCystatin) and to determine the mechanism underlying these effects.METHODS: The releases of IL-10 and IL-12, and the expression of macrophage subtype markers LIGHT (M2b) and Arg-1 (M2a+M2c) were assessed by ELISA, RT-qPCR and Western blot. The phosphorylation level of AKT was assessed by Western blot.RESULTS: SjCystatin promoted the continuous increase in IL-10 level at 6 h, 12 h and 24 h, and increased the amount of mRNA and protein expression of LIGHT, but down-regulated the amount of mRNA and protein expression of AKT. The addition of PI3K/AKT inhibitor reduced the release of IL-10 at 12 h and 24 h, reduced the mRNA and protein expression of LIGHT at 24 h, up-regulated the mRNA and protein expression of Arg-1 at 24 h, and decreased the phosphorylation level of AKT.CONCLUSION: SjCystatin promotes the differentiation of M2 macrophage to M2b macrophage subtype, and the PI3K/AKT signaling pathway is involved in this process. |
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Keywords: | Cystatin M2b macrophages M2 macrophages PI3K/AKT signaling pathway |
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